Culture medium for elaeagnus angustifolia tissue culture for environment protection and method
A tissue culture and environmental protection technology, applied in the field of plant tissue culture, can solve the problems of no process and formula, etc., and achieve the effect of convenient and rapid reproduction
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[0029] Example one
[0030] A medium for tissue culture of Elaeagnus jujube for environmental protection, including three types: primary culture medium, proliferation medium and rooting medium.
[0031] Primary medium formula: 1 / 2MS medium, kinetin 0.5mg / L, sucrose 25g / L, agar 3g / L; when preparing, first measure MS medium, add kinetin, add sucrose, and wait for sucrose to dissolve, Adjust the pH to 5.8-6.0, add agar, heat to melt the agar, autoclave at 121°C for 25 minutes, and cool to solid for later use.
[0032] Proliferation medium formula: MS medium, kinetin 1.0mg / L, indole acetic acid 0.5mg / L, sucrose 25g / L, agar 3g / L; when preparing, first measure MS medium and add kinetin and indole Acetic acid, add sucrose, after the sucrose is dissolved, adjust the pH to 5.8-6.0, add agar, heat to melt the agar, autoclave at 121°C, 25 minutes, and cool to solid for later use;
[0033] Rooting medium formula: 1 / 2MS medium, kinetin 0.2mg / L, indoleacetic acid 1.0mg / L, sucrose 25g / L, agar 3g / L;...
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[0039] Example two
[0040] The difference from the first embodiment is that the first-generation buds in step two are lateral buds.
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[0041] Example three
[0042] The difference from the second embodiment is that the first-generation buds in step two take the top buds.
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