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Method and application for reducing PAES pollution in plants by using plant endophytes

A technology of plant endophytes and plant bodies, applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of reducing PAEs, etc., and achieve low accumulation, low cost, and simple operation Effect

Active Publication Date: 2019-11-19
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as far as we know, there are no patents and literature reports on the application of plant endophytes to reduce PAEs in crops

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] After activating Bacillus megaterium YJB3 on LB medium for 8 hours, transfer it to LB liquid medium, culture it on a shaker for 7 hours, rotate at 100-150 rpm, cultivate at 30°C, and collect bacteria by centrifugation at 3000 rpm, then wash with phosphate buffer for 3 hours. After that, resuspend with phosphate buffer solution containing 0.5-1.8% sodium acetate solution, and adjust the cell concentration to 10 6 ~10 9 CFU / mL.

Embodiment 2

[0033] Using plant endophytes to eliminate PAEs in plants, inoculate Bacillus megaterium YJB3 on plant seeds, first spray the full-grained plant seeds with 3% H 2 o 2 Rinse for 3 minutes, rinse with sterile water for 3 times, rinse with 70% alcohol for 5 minutes, rinse with sterile water for 4 times, and air dry; then soak in 0.05mol / L KOH solution for 12 minutes, rinse with sterile distilled water for 3 times, or soak in chloroform 20s, or treated with cuticle enzyme (100μg / mL) for 20min at room temperature, remove the seed coat wax and cuticle, rinse 4 times with sterile distilled water, and dry naturally; then at room temperature, soak the pretreated plant seeds in 10 7 CFU / mL bacterial suspension, soak for 18h. Let the cabbage seeds inoculated with Bacillus megaterium YJB3 be sown in the soil containing DBP (initial concentration is 100mg / kg), the bacterial strain YJB3 colonizes the plant body through the plant seed approach, harvest the plants after cultivating in the gr...

Embodiment 3

[0035] Set the concentration to 10 8 The bacterial suspension of CFU / mL Bacillus megaterium YJB3 was applied to the leaf surface of the soil culture plant ryegrass, once every 3 hours, for 4 consecutive times, and the young leaves near the top of the plant were selected. And before applying, first rinse the plant leaves with sterile water 2 to 3 times to remove the plant leaf dust. The initial concentration of DEP in the soil was 100mg / kg, and it was harvested after 55 days of cultivation in the greenhouse. The DEP contents in the shoots and roots of the plants were measured respectively. Compared with the uninoculated plants, the DEP contents in the shoots and roots of the inoculated plants decreased by 38.3% and 18.3% respectively. .

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Abstract

The invention discloses a method and application of utilizing plant endophyte to reduce pollution of PAEs in plants and belongs to the field of soil organic pollution bioremediation and agricultural product safety. In the method and application, the plant endophyte bacillus megaterium YJB3 is colonized in the plants by means of soaking seeds, soaking roots, foliage spraying or smearing, roots irrigating and the like, and then the processed plant seeds are sown in the PAEs polluted soil, or plant seedlings that have been inoculated with bacillus megaterium YJB3 are water-cultured in nutrient solution containing PAEs or planted in soil polluted by PAEs, and accumulation of PAEs in plants is reduced. The method and application have the advantages of simple operation, safety and economy, and that secondary pollution to the environment is not caused and PAEs accumulated in the plants is effectively removed and a risk of agricultural product pollution is avoided, which is of great significance in producing safe agricultural products by effectively utilizing medium and low concentration polluted soil and achieving 'production, while repairing'.

Description

technical field [0001] The invention belongs to the fields of bioremediation of environmental organic pollution and safety of agricultural products, and in particular relates to a method and application for reducing phthalate (PAEs) pollution in plants by using endophytic bacteria in plants. Background technique [0002] Phthalates (PAEs) are widely used in the plastics industry mainly as plasticizers, and can also be used as additives in interior decoration materials, building materials, clothing, pesticides and fertilizers. The consumption of PAEs is about 600,000 to 800,000 tons per year. Due to the wide application of products containing PAE, it has been detected in various environmental media. PAEs are a class of environmental hormone pollutants, some of which have strong endocrine disruption, reproductive and developmental toxicity, hepatotoxicity and even "three-induced" effects. Daily dietary intake of agricultural products containing PAEs leads to long-term low-do...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/00A01G31/00A01G7/06A01C1/00C12N1/20C12R1/11
CPCA01C1/00A01G7/06A01G9/0299A01G22/00A01G31/00C12N1/20
Inventor 莫测辉冯乃宪李彦文喻娇蔡全英李慧洪芸
Owner JINAN UNIVERSITY
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