Bacillus amyloliquefaciens and application thereof to preparation of microbial agent for controlling radix rehmanniae root rot and spot blight

A technology for dissolving starch spores and microbial strains is applied in the field of microbial control of plant diseases, and can solve problems such as increasing the hidden danger of Rehmannia glutinosa, infection of Rehmannia glutinosa, and environmental pollution.

Inactive Publication Date: 2017-12-22
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The growth period of Rehmannia glutinosa is long, usually from April to December. From June to August every year, the hot and rainy weather in Jiaozuo City, Henan Province often causes the outbreak of some fungal pathogenic microorganisms such as root rot and spot blight pathogens Proliferate and infect rehmannia root tubers, resulting in large-scale production reduction
In order to achieve the stable production of Rehmannia glutinosa, a large number of pesticides are sprayed every year to control the root rot and spotted blight of Rehmannia glutinosa, but the residues of these pesticides pollute the environment on the one hand, and increase the safety of rehmannia glutinosa as a drug on the other hand. Hidden danger

Method used

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  • Bacillus amyloliquefaciens and application thereof to preparation of microbial agent for controlling radix rehmanniae root rot and spot blight
  • Bacillus amyloliquefaciens and application thereof to preparation of microbial agent for controlling radix rehmanniae root rot and spot blight

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Experimental program
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Effect test

Embodiment 1

[0015] Embodiment 1 (antagonistic rehmannia root rot)

[0016] Adopt plate confrontation method, will cultivate the rehmannia root rot pathogen of 4-5 days on the PDA plate Fusarium solani Break into a bacterium cake with a diameter of 6mm, inoculate it in another PDA medium with a pore size of 6 mm, and then inoculate the purified Bacillus amyloliquefaciens ZSY-1 activated for 36 hours on the LB medium at a distance of 2 cm from the bacterium cake. Each PDA plate was symmetrically inoculated with 4 points, repeated 3 groups, and the point was connected with sterile water as a blank control, and placed in a 28 ° C incubator. After the pathogenic bacteria in the control grew for 4 days, the radius of the pathogenic bacteria colony was measured. According to the inhibition rate = ( Blank control pathogenic bacteria colony radius - ZSY-1 confrontation pathogenic bacteria radius) / blank control pathogenic bacteria colony radius * 100% to calculate the antibacterial effect. The ...

Embodiment 2

[0017] Embodiment 2 (antagonistic macular blight)

[0018] Using the flat plate confrontation method, the ground yellow spot blight bacteria that will be cultivated on the PDA plate for 4-5 days Septoria digitalis pass Break into a bacterium cake with a diameter of 6mm, inoculate it in another PDA medium with a pore size of 6 mm, and then inoculate the purified Bacillus amyloliquefaciens ZSY-1 activated for 36 hours on the LB medium at a distance of 2 cm from the bacterium cake. Each PDA plate was symmetrically inoculated with 4 points, repeated 3 groups, and the point was inoculated with sterile water as a blank control, placed in a 28°C incubator, and after the pathogenic bacteria in the control grew for 4 days, according to the inhibition rate = (blank control pathogenic bacteria Colony radius - ZSY-1 confrontation pathogenic bacteria radius) / blank control pathogenic bacteria colony radius * 100% to calculate the antibacterial effect. The results showed that Bacillus ...

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Abstract

The invention discloses bacillus amyloliquefaciens and application of the bacillus amyloliquefaciens to preparation of a microbial agent for controlling radix rehmanniae root rot and spot blight, and belongs to the technical field of microbial control of plant diseases. Through the technical scheme, the bacillus amyloliquefaciens is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation umber is CGMCC NO. 14229. The invention also specifically discloses a separation and purification method of the bacillus amyloliquefaciens and application of the bacillus amyloliquefaciens to preparation of the microbial agent for controlling radix rehmanniae root rot and spot blight. The bacteria is obtained from rhizosphere soil of famous and precious traditional Chinese medicine such as radix rehmanniae by using a separation and purification method of a flat plate, the bacteria is authenticated into the bacillus amyloliquefaciens through 16s rDNA, and the bacteria can be used for efficiently resisting radix rehmanniae root rot and spot blight, so that a new culture resource is provided for developing the biocontrol microbial agent of radix rehmanniae.

Description

technical field [0001] The invention belongs to the technical field of microbial control of plant diseases, and in particular relates to a strain of bacillus amyloliquefaciens and its application in the preparation of microbial agents for preventing and controlling rehmannia root rot and spot blight. Background technique [0002] Rehmannia glutinosa root is a traditional Chinese medicine with great demand. However, Rehmannia glutinosa itself has strict requirements on growth conditions. It likes dryness but avoids water. The produced Rehmannia glutinosa is of the best quality), which makes it a rare economical herb. The growth period of Rehmannia glutinosa is long, usually from April to December. From June to August every year, the hot and rainy weather in Jiaozuo City, Henan Province often causes the outbreak of some fungal pathogenic microorganisms such as root rot and spot blight pathogens Proliferate and infect rehmannia root tubers, resulting in large-scale yield reduc...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/02C12N1/205C12R2001/07
Inventor杨清香王瑞飞王妍李明军
OwnerHENAN NORMAL UNIV