Method for controlling inoculation force of solid culture media by aid of damping force and sensor technologies

Abstract

The invention discloses a method for controlling inoculation force of solid culture media by the aid of damping force and sensor technologies. The method is implemented by the aid of an inoculation loop damping and sensor detection assembly, an inoculation loop rotary driven shaft, an inoculation loop rotary driven wheel, a synchronous belt, an inoculation loop rotary motor mounting component, an inoculation loop rotary driving wheel, an inoculation loop rotary motor, an inoculation loop rotary component, a switching component, a Z-axis slide table module, a base plate, a culture dish and the culture media. The inoculation loop damping and sensor detection assembly comprises an inoculation loop mounting component, and two inoculation loop mechanisms are arranged on the inoculation loop mounting component and comprise inoculation loops, inoculation loop switching components a, inoculation loop switching components b, inoculation damping springs and inoculation sensing adjusting screws. The method has the advantages that force applied to the surfaces of the culture media when the inoculation loops are in contact with the surfaces of the culture media in a pressure manner can be guaranteed; the inoculation loops can be assuredly in contact with the surfaces of the culture media in inoculation procedures; the service cost can be reduced to a great extent; poor scribing effects because of jumping of the inoculation loops due to high-speed scribing can be prevented.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for controlling the inoculation strength of a solid medium by using damping force and sensor technology. Background technique [0002] Microorganisms are an eternal topic of bacterial drug resistance. In recent years, the mutation of avian influenza virus is the most obvious. The indiscriminate use of antibiotics in China is serious, which leads to the continuous generation of drug-resistant mutations of bacteria and viruses and new species. In the past two years, the country The Health and Family Planning Commission clearly stipulates that hospitals at all levels need to pay attention to microorganisms, and they need to increase their efforts in the detection of bacteria and fungi. For patients who need to be treated with antibiotics, more than 50% of the patients must be required to conduct bacterial culture experiments (vaccination is required). [0003] At present, the m...

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Problems solved by technology

[0010] (2) Since the equipment needs to continuously sterilize the inoculation loop at high temperature during the inoculation process, a long cooling time is required during the inoculation process, the efficiency will be relatively low, and the applicability to hospitals with large sample volumes is too poor;

[0012] (4) The inoculation loop of this equipment is easily deformed during long-term high-temperature sterilization, but the equipment itself does not have any detection mechanism to compensate for the position difference caused by the deformation of the inoculation loop. The phenomenon of scratching the medium, resulting in abnormal inoculation results

[0014] (1) This equipment uses the gravity marking method for inoculation. During the actual inoculation, only the petri dish is moving. During the sampling process of the inoculation loop, the inoculation loop needs to be rotated from the sampling position to the marking position. There is a risk of sample dripping (the cam mechanism has a large impact force), and the sample inoculated on the petri dish will become very small after the sample drips, which has a particularly large impact on the experimental results;

[0015] (2) This equipment relies on gravity streaking for inoculation, relying on the inoculation loop floating on the surface of the medium. During high-speed inoculation, the inoculation loop will jump on the surface of the medium, and the inoculation effect is not good;

[0016] (3) The floating effect of the inoculation loop on the surface of the medium depends on the overpressure of the inoculation loop to ensure the floating effect on the surface of the medium, but the actual inoculation loop is bent, and if the amount of bending is too large, it will also lead to the phenomenon that the medium cannot be touched

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