A composition for improving the stability of troponin antibody in latex immunoturbidimetric method

A troponin and latex immunization technology, applied in the field of troponin antibody, can solve problems such as poor stability, unfavorable in vitro diagnosis, accuracy, linearity, and poor precision, and achieve small interference, protection of biological functions, and easy operation Effect

Active Publication Date: 2020-02-07
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antibody coupled to latex microspheres has poor stability, resulting in poor accuracy, linearity, precision and other indicators, which is not conducive to using it for in vitro diagnosis

Method used

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  • A composition for improving the stability of troponin antibody in latex immunoturbidimetric method
  • A composition for improving the stability of troponin antibody in latex immunoturbidimetric method
  • A composition for improving the stability of troponin antibody in latex immunoturbidimetric method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Screen the buffer type of the composition: prepare 25mM Tris-HCl buffer, HEPES buffer, and phosphate buffer at pH 7.4 respectively. Detect its influence on the accuracy of theoretical concentration such as 5ug / L, 10ug / L, 20ug / L; the test results are shown in the table below.

[0020] name 5(ug / L) 10(ug / L) 20(ug / L) Tris-HCl buffer 4.5 9.3 19.9 HEPES buffer 4.1 11.5 21.7 Phosphate buffer 4.9 10.1 19.8

[0021] Through the comprehensive comparison of the test results, the difference between the actual concentration and the theoretical concentration of the low value, middle value and high value of the target substance detected in the three buffer solutions is calculated, and the difference of the phosphate buffer solution is small and their changes are average. less than 2%.

Embodiment 2

[0023] Screening is carried out for the pH value of the definite buffer of this composition: respectively configure 25mM, pH6.0 phosphate buffer, pH6.5 phosphate buffer, pH7.4 phosphate buffer, detect its to theoretical concentration accuracy The impact is 5ug / L, 10ug / L, 20ug / L; the test results are shown in the table below.

[0024] name 5(ug / L) 10(ug / L) 20(ug / L) pH6.0 4.6 10.3 20.9 pH6.5 4.5 11.1 18.7 pH7.4 4.8 10.5 20.3

[0025] Through the comprehensive comparison of the test results, the difference between the actual concentration and the theoretical concentration of the low, middle and high values ​​of the target substance detected in the three buffer solutions with different pH values, the difference of the phosphate buffer solution with pH 7.4 is calculated The changes are small and they all vary by less than 5%.

Embodiment 3

[0027] Screening for the concentration of the determined buffer of the composition: configure 10mM, 25mM, and 50mM phosphate buffer respectively, and detect its influence on the accuracy of the theoretical concentration, such as 5ug / L, 10ug / L, and 20ug / L; test results See the table below.

[0028]

[0029]

[0030] Through the comprehensive comparison of the test results, the difference between the actual concentration and the theoretical concentration of the low, middle and high values ​​of the target substance detected in the buffer solutions of three different concentrations can be calculated to calculate the difference and change of the phosphate buffer solution with pH 7.4 small and their variation is less than 10%.

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Abstract

The invention discloses a composition for improving the stability of a troponin antibody in latex immunoturbidimetry. The composition provided by the invention is prepared from a 25mM phosphate buffer solution with pH (potential of Hydrogen) of 7.4, 0.025 percent of potassium chloride, 0.01 percent of magnesium chloride, 0.05 percent of gelatin, 1 percent of trehalose, 0.2 percent of mannitol and 7 percent of glycerol. The composition is used for improving the stability of the troponin antibody in the latex immunoturbidimetry and protecting the biological function of the troponin antibody and does not interfere in a reaction process in which the troponin antibody participates; the absorbance value of the composition is 0.001 to 0.003. The composition is simple in component, better in protection effect and low in own background value, and can be also widely applied to other detection reagents and a scientific basis is provided for improving the stable performance of a detection reagent.

Description

technical field [0001] The invention relates to the technical field of troponin antibodies, in particular to a composition for improving the stability of troponin antibodies in latex immunoturbidimetry. Background technique [0002] Latex immunoturbidimetry is a simple and fast detection technology, which is suitable for automatic biochemical analyzers. During the reaction, the latex particles coupled with the antibody are combined with the target object, and due to the change of turbidity in the reaction system, corresponding detection results are generated. Signal. Improving the stability of its antibody is the key to determining the performance index of the kit. Troponin is an important functional protein of cardiomyocytes and a sensitive and specific biological indicator of myocardial injury. However, the antibody coupled to latex microspheres has poor stability, resulting in poor accuracy, linearity, precision and other indicators, which is not conducive to using it f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/544
CPCG01N33/54313G01N33/544G01N33/6887
Inventor 王贤俊何丹
Owner 王贤俊
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