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Method of delaying eclosion of Aedes albopictus pupae and method of transporting Aedes albopictus pupae

A technology for Aedes albopictus and mosquito pupae, applied in the field of mosquito control, can solve the problems of short emergence time, high transportation cost, death of adult mosquitoes, etc., and achieve the effects of reducing transportation cost, convenient operation, and reducing transportation space.

Active Publication Date: 2020-09-11
GUANGZHOU WOLBAKI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, with the continuous deepening of research on mosquito ecology and control technology, especially the in-depth development of wolbachia technology, the release of male mosquitoes carrying wolbachia, that is, "mosquito control with mosquitoes", has become the most cutting-edge control method at present. Therefore, in the experiment When the target male mosquitoes can be mass-produced in laboratories and workshops, a reasonable transportation and release method is an important factor for the smooth implementation of "killing mosquitoes with mosquitoes". However, there are few research reports on the transportation and release of male mosquitoes and pupae at home and abroad.
[0004] The male pupae of Aedes albopictus begins to emerge about 45 hours after pupae, but the separation time of male and female is generally 35-40 hours after pupae, and the time to eclosion is very short, so the long-distance transportation of Aedes albopictus pupae cannot be realized
Therefore, the existing transportation and release methods are to transport adult mosquitoes and release adult mosquitoes. The problem with this method is that a small space will cause a large number of adult mosquitoes to die after emergence, so a large space is required for storage and transportation volume, resulting in high transportation costs. , it is difficult to popularize and apply in the case of large-scale release

Method used

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  • Method of delaying eclosion of Aedes albopictus pupae and method of transporting Aedes albopictus pupae
  • Method of delaying eclosion of Aedes albopictus pupae and method of transporting Aedes albopictus pupae
  • Method of delaying eclosion of Aedes albopictus pupae and method of transporting Aedes albopictus pupae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example studies the effects of different storage temperatures on the emergence and survival of Aedes albopictus pupae.

[0031] Appliances and materials: 30*30*30cm adult mosquito breeding cage, 10*10cm square petri dish, biochemical incubator, purified water, male Aedes albopictus pupae, disposable lunch box.

[0032] Quantity used: 12 adult mosquito breeding cages, 12 petri dishes, 3 biochemical incubators, and 12,000 male mosquito pupae.

[0033] Experimental method and results: Each 1000 male mosquito pupae was regarded as a sample and placed in a petri dish; 12000 male mosquito pupae were randomly divided into 4 groups and numbered, and three parallel samples were repeated for each group. The numbered petri dishes were respectively placed in biochemical incubators with different temperatures (set according to Table 1), and the humidity in each group of biochemical incubators was controlled to be 80%. Group 1 is a blank control group, directly put male mosquit...

Embodiment 2

[0052] This example studies the effects of storage temperature at 18°C ​​and different storage times on the emergence and survival of Aedes albopictus pupae.

[0053] Appliances and materials: 30*30*30cm adult mosquito breeding cage, 10*10cm square petri dish, biochemical incubator, purified water, male Aedes albopictus pupae, disposable lunch box.

[0054]Quantity used: 12 adult mosquito breeding cages, 12 petri dishes, 3 biochemical incubators, and 12,000 male mosquito pupae.

[0055] Experimental method and results: Each 1000 male mosquito pupae was regarded as a sample and placed in a petri dish; 12000 male mosquito pupae were randomly divided into 4 groups and numbered, and three parallel samples were repeated for each group. The numbered culture dishes were placed in the biochemical incubator respectively, and the temperature in the biochemical incubator of each group was controlled to be 18° C. and the humidity was 80%. The specific storage time of each group is as fol...

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Abstract

The invention relates to a method for delaying eclosion of aedes albopictus pupas and a method for transporting aedes albopictus pupas. The method for delaying eclosion of aedes albopictus pupas comprises the following step: preserving aedes albopictus pupas in an environment of 16-21 DEG C. Tests show that if the aedes albopictus pupas are preserved at 16-21 DEG C, the time from nymphosis to eclosion is prolonged, normal eclosion can be still achieved for the preserved aedes albopictus pupas, and the aedes albopictus pupas and the number of dead mature misquotes after eclosion are not greatlydifferent from those of room-temperature eclosion. In the transportation process of the aedes albopictus pupas, the temperature of the transportation environment is kept within 16-21 DEG C, then theaedes albopictus pupas can be prevented from eclosion in the transportation process, remote-distance transportation of the aedes albopictus pupas can be achieved, the transportation space can be greatly saved, the transportation cost can be reduced, and large-scale transportation of factories is powerfully ensured.

Description

technical field [0001] The invention relates to the technical field of mosquito control, in particular to a method for delaying emergence of Aedes albopictus pupae and a method for transporting Aedes albopictus pupae. Background technique [0002] The use of artificial breeding of insects is one of the basic techniques for studying entomology. It can breed certain types of insects without seasonal restrictions for research on insect biology, physiology and the formulation of integrated pest management measures. Mosquito, Belonging to the family Insecta Diptera, there are about 3000 species in the world. It is a tiny flying insect with piercing-sucking mouthparts. Usually female mosquitoes feed on blood, while male mosquitoes suck the sap of plants. Blood-sucking female mosquitoes are intermediate hosts for dengue, malaria, yellow fever, filariasis, Japanese encephalitis, and other pathogens. [0003] At present, with the continuous deepening of research on mosquito ecolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02B65D81/18B65D85/50
Inventor 不公告发明人
Owner GUANGZHOU WOLBAKI BIOTECH CO LTD