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Maize Molecular Markers and Its Application in Detection of Maize Flowering-related Traits

A technology of flowering period and maize, which is applied in the application field of detecting the related characters of maize flowering period, and can solve the problems such as that the development of maize molecular markers has not reached saturation and the like.

Active Publication Date: 2020-12-01
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although a large number of molecular markers have been developed based on the maize genome, the development of maize molecular markers has not yet reached saturation

Method used

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  • Maize Molecular Markers and Its Application in Detection of Maize Flowering-related Traits
  • Maize Molecular Markers and Its Application in Detection of Maize Flowering-related Traits
  • Maize Molecular Markers and Its Application in Detection of Maize Flowering-related Traits

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Embodiment 1

[0052]Example 1. Maize molecular markers can be used to detect traits related to the flowering period of maize

[0053]The corn molecular marker provided in this example is a DNA fragment shown at positions 42-401 of sequence 1 in the sequence listing.

[0054]The primer pair M1 is designed according to the upstream and downstream of the maize molecular marker in the maize genome. The primer pair M1 consists of the single-stranded DNA shown in the 1st-19th position of the sequence 1 in the sequence table and complementary to the 482-501th position of the sequence 1 in the sequence table. Composed of single-stranded DNA.

[0055]Use primer pair M1 to perform PCR amplification on genomic DNA of different maize. There are three types of PCR products. The sequence of the first PCR product is sequence 1, the sequence of the second PCR product is sequence 2, and the sequence of the third PCR product is There are both sequence 1 and sequence 2. According to the sequence of PCR products, corn is div...

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Abstract

The invention discloses a maize molecular marker and application thereof to detection of relevant characters of the blossoming period of maize. The maize molecular marker is (A1) or (A2) or (A3), wherein (A1) is a DNA fragment expressed as the sites 42-401 in a sequence 1 of a sequence table; (A2) is a DNA fragment obtained by substituting and / or deleting and / or adding one or several nucleotides of the DNA fragment of (A1); the homoousia between the DNA fragment of (A2) and the nucleotide sequence defined by (A1) is 90% or is greater than 90%; (A3) comprises the DNA fragment of the (A1) or (A2). Experiments prove that the blossoming period, the plant height and the leaf number of the maize with two chromosomes which contain the DNA fragment expressed as the sites 42-401 in the sequence 1 of the sequence table are obviously less than that of the maize with two chromosomes which do not contain the DNA fragment expressed as the sites 42-401 in the sequence 1 of the sequence table, so thatthe experiments show that the maize molecular marker can be used for identifying the blossoming period, the plant height and the leaf number of the maize.

Description

Technical field[0001]The invention relates to a corn molecular marker in the field of biotechnology and its application in detecting related traits of corn flowering period.Background technique[0002]Flowering is an important process in the transformation of plants from vegetative growth to reproductive growth. When the plant grows and develops to a certain period of time, under suitable light, temperature, moisture and nutrient conditions, the florigen signal produced by the plant leaves is transported to the stem apical meristem through the vascular bundle, which induces the expression of related flower-forming genes and differentiates Flower primordium emerges and further develops into floral organs. Flowering period is one of the important agronomic traits of crops. The length of flowering period determines the length of crop growth period, and to a certain extent restricts the regional and seasonal adaptability of crops. Plant height and leaf number as related traits of flowerin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 田丰黄成
Owner CHINA AGRI UNIV
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