Maize molecular marker and application thereof to detection of relevant characters of blossoming period of maize

A molecular marker, flowering stage technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem that the development of maize molecular markers has not reached saturation and so on

Active Publication Date: 2018-01-16
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although a large number of molecular markers have been developed based on the m

Method used

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  • Maize molecular marker and application thereof to detection of relevant characters of blossoming period of maize
  • Maize molecular marker and application thereof to detection of relevant characters of blossoming period of maize
  • Maize molecular marker and application thereof to detection of relevant characters of blossoming period of maize

Examples

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Embodiment 1

[0052] Example 1. Maize molecular markers can be used to detect traits related to the flowering period of maize

[0053] The maize molecular marker provided in this example is the DNA fragment shown in the 42-401 position of Sequence 1 in the Sequence Listing.

[0054] The primer pair M1 is designed according to the upstream and downstream of the corn molecular markers in the corn genome, and the primer pair M1 is composed of the single-stranded DNA shown in the 1-19th position of the sequence 1 in the sequence listing and complementary to the 482-501th positions of the sequence 1 in the sequence listing single-stranded DNA composition.

[0055] Using the primer pair M1 to perform PCR amplification on the genomic DNA of different corns, there are three types of PCR products, the sequence of the first PCR product is sequence 1, the sequence of the second PCR product is sequence 2, and the sequence of the third PCR product There is both sequence 1 and sequence 2. According to ...

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Abstract

The invention discloses a maize molecular marker and application thereof to detection of relevant characters of the blossoming period of maize. The maize molecular marker is (A1) or (A2) or (A3), wherein (A1) is a DNA fragment expressed as the sites 42-401 in a sequence 1 of a sequence table; (A2) is a DNA fragment obtained by substituting and/or deleting and/or adding one or several nucleotides of the DNA fragment of (A1); the homoousia between the DNA fragment of (A2) and the nucleotide sequence defined by (A1) is 90% or is greater than 90%; (A3) comprises the DNA fragment of the (A1) or (A2). Experiments prove that the blossoming period, the plant height and the leaf number of the maize with two chromosomes which contain the DNA fragment expressed as the sites 42-401 in the sequence 1 of the sequence table are obviously less than that of the maize with two chromosomes which do not contain the DNA fragment expressed as the sites 42-401 in the sequence 1 of the sequence table, so thatthe experiments show that the maize molecular marker can be used for identifying the blossoming period, the plant height and the leaf number of the maize.

Description

technical field [0001] The invention relates to the field of biotechnology, a molecular marker of maize and its application in detecting traits related to the flowering period of maize. Background technique [0002] Flowering is an important process in the transition of plants from vegetative to reproductive growth. When the plant grows and develops to a certain period, under the appropriate light, temperature, water and nutrient conditions, the florigen signal produced by the plant leaves is transported to the shoot apical meristem through the vascular bundle, and induces the expression of related flower-forming genes, and then differentiates. The flower primordium develops further to form floral organs. Flowering period is one of the important agronomic traits of crops. The length of flowering period determines the length of crop growth period, and to a certain extent, restricts the regional and seasonal adaptability of crops. Plant height and leaf number, as the related...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 田丰黄成
Owner CHINA AGRI UNIV
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