Maize molecular marker and application thereof to detection of relevant characters of blossoming period of maize
A molecular marker, flowering stage technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem that the development of maize molecular markers has not reached saturation and so on
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[0052] Example 1. Maize molecular markers can be used to detect traits related to the flowering period of maize
[0053] The maize molecular marker provided in this example is the DNA fragment shown in the 42-401 position of Sequence 1 in the Sequence Listing.
[0054] The primer pair M1 is designed according to the upstream and downstream of the corn molecular markers in the corn genome, and the primer pair M1 is composed of the single-stranded DNA shown in the 1-19th position of the sequence 1 in the sequence listing and complementary to the 482-501th positions of the sequence 1 in the sequence listing single-stranded DNA composition.
[0055] Using the primer pair M1 to perform PCR amplification on the genomic DNA of different corns, there are three types of PCR products, the sequence of the first PCR product is sequence 1, the sequence of the second PCR product is sequence 2, and the sequence of the third PCR product There is both sequence 1 and sequence 2. According to ...
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