Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Thromboelastography quality control product and preparation method thereof

A quality control product and blood plasma technology, which is applied in the preparation of test samples, material inspection products, biological testing, etc., can solve the problems of high cost and complicated preparation steps, and achieve the effect of low cost, simplified preparation steps and accurate results

Active Publication Date: 2021-10-22
上海原科实业发展有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In order to solve the problem of complex preparation steps and high cost caused by the need to extract blood coagulation factors in the preparation process of quality control products in the prior art, the object of the present invention is to provide a quality control product for thromboelastography. For this reason, the present invention will also provide a A kind of preparation method of thrombelastography instrument quality control product

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Thromboelastography quality control product and preparation method thereof
  • Thromboelastography quality control product and preparation method thereof
  • Thromboelastography quality control product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] 1. Preparation of Quality Control Products

[0034] Include the following steps:

Embodiment 1

[0036] S1, collect 3000g fresh sheep whole blood, add 76g sodium citrate anticoagulant, centrifuge at 3000rpm / min for 10 minutes to obtain 1800g plasma;

[0037] S2, collect 2000g fresh sheep whole blood, add 76g sodium citrate anticoagulant, centrifuge at 3000rpm / min for 30 minutes to obtain 1200g serum;

[0038] S3, divide 1800g of plasma obtained in S1 into 3 parts, namely 600g of the first part, 400g of the second part and 800g of the third part;

[0039] S4, the first part of plasma was centrifuged at 3000rpm / min for 10 minutes to obtain 350g of factor-rich sheep plasma as the lower layer of plasma,

[0040] The measured R is 0.8min, K is 0.7min, Angle is 81.5, and MA value is 76.4mm;

[0041] S5, add 24g of barium sulfate to the second part, mix well, and centrifuge to obtain 240g of factor-deficient sheep plasma in the upper layer,

[0042] And measured R is 4.8min, Angle is 72.4, MA value is 40.3mm;

[0043] S6, freeze the third part at -80°C for 6 hours, and thaw at ...

Embodiment 2

[0049] S1, collect 3000g fresh sheep whole blood, add 76g sodium citrate anticoagulant, centrifuge at 3000rpm / min for 10 minutes to obtain 1800g plasma;

[0050] S2, collect 2000g fresh sheep whole blood, add 76g sodium citrate anticoagulant, centrifuge at 3000rpm / min for 30 minutes to obtain 1200g serum;

[0051] S3, divide 1800g of plasma obtained in S1 into 3 parts, namely 600g of the first part, 400g of the second part and 800g of the third part;

[0052] S4, the first part of plasma was centrifuged at 3000rpm / min for 10 minutes to obtain 350g of factor-rich sheep plasma as the lower layer of plasma,

[0053] The measured R is 0.8min, K is 0.7min, Angle is 81.5, and MA value is 76.4mm;

[0054] S5, add 24g of barium sulfate to the second part, mix well, and centrifuge to obtain 240g of factor-deficient sheep plasma in the upper layer,

[0055] And measured R is 4.8min, Angle is 72.4, MA value is 40.3mm;

[0056] S6, freeze the third part at -80°C for 6 hours, and thaw a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a quality control product, comprising a quality control product 1 and a quality control product 2, characterized in that, the quality control product 1 comprises the following components: 2-6 parts by weight of factor-rich sheep plasma, 2-5 parts by weight The goat serum in parts by weight and the fibrinogen mother liquor in 1-4 parts by weight; the quality control product 2 includes the following components: 1-4 parts by weight of factor-deficient goat plasma, 5-10 parts by weight of goat serum and 0.3- 2 parts by weight of fibrinogen mother solution, the fibrinogen mother solution is prepared by dissolving fibrinogen in goat serum, wherein the mass percentage of fibrinogen is 10%; There is no need to extract coagulation factors, the preparation steps are simplified, the operation is easy, the cost is low, the results are accurate, and the stability is good; Replacing the quality control products produced by the listed American Haemoscope company, reducing the cost of use for customers.

Description

technical field [0001] The invention relates to the field of quality control of blood coagulation detection items of a clinical thromboelastography instrument, in particular to a quality control product of a thromboelastography instrument and a preparation method thereof. Background technique [0002] Thromboelastography is used to monitor the coagulation state of blood samples to assist clinical evaluation of patients. Thromboelastography is widely used in the monitoring of postoperative bleeding and / or thrombosis during or after cardiac surgery, organ transplantation, tumor and radiotherapy. [0003] Thromboelastography uses a coagulation method to simulate physiological blood coagulation conditions. Some reagent is added to start the blood coagulation reaction, so that the fibrinogen in the sample is converted into cross-linked fibrin, and the sample coagulates. The motor inside the instrument drives the sample cup carrying the blood sample to rotate at a certain angle ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/42G01N33/86
Inventor 王连升
Owner 上海原科实业发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com