Preparation method and application of a kind of poultry compound probiotic feed

A technology of compound probiotics and poultry, which is applied in the field of poultry breeding, can solve problems such as abuse, achieve the effects of improving nutritional levels, reducing the use of antibiotics, and improving breeding benefits

Inactive Publication Date: 2021-06-15
广东科贸职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the problems of abuse of antibiotics in the existing poultry breeding, and provide a strain of Lactobacillus bulgaricus P-3, which can be added to the feed as a feed additive, and has the functions of improving the intestinal health of poultry and improving the health of poultry. Advantages of resistance and growth promotion of poultry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Select 10 15-day-old healthy pigeons, first kill the normal pigeons, operate aseptically, scrape the intestinal and crop contents of the pigeons with an inoculation loop and put them in sterile normal saline, and then use the inoculation loop to draw the pigeons continuously. Line inoculated into MRS medium, placed at 36 ℃, anaerobic culture for 48 hours, select suspected lactic acid bacteria on the plate, purified, and then streak inoculated into MRS and MC medium, MRS medium 36 ℃ anaerobic culture for 48 hours, MC medium Aerobic culture at 36°C for 48h, after culture, the colony characteristics were observed. According to "Lactic Acid Bacteria Classification and Identification and Experimental Methods" and "Common Bacteria System Identification Manual", the morphological characteristics and physiological and biochemical identification of the isolated strains were carried out.

[0025] After anaerobic cultivation of the isolated and purified lactic acid bacteria on MRS...

Embodiment 2

[0029] Preparation of poultry feed:

[0030] (1) Preparation of fermentation broth: water heating temperature to 40~50℃, add 2~8wt% food grade glucose, add 0.1~0.5wt% lactic acid bacteria, 0.1~0.5wt% yeast, 0.1~0.5wt% bacillus , stir to dissolve, cultivate for 18-24h,

[0031] (2) Preparation of culture medium (materials): soybean meal, corn, broken rice are crushed and sieved for use.

[0032] (3) Inoculation: After testing, the fermentation broth can be inoculated with PH≤4.0. The crushed and sieved soybean meal 10-25%, corn 10-25%, and broken rice 10-30% are stirred in a mixer for 3-6 minutes. (1) 30-45% of the obtained fermented liquid, stir for 1-5 min, and pack after mixing evenly.

[0033] (4) Fermentation: The packaged and sealed culture is sent to the fermentation workshop and fermented at a constant temperature of 25-35°C for 3 days.

[0034] (5) Acceptance of fermentation materials: After testing, PH≤4.5, lactic acid bacteria ≥1 billion cfu / g, Bacillus ≥1 billion...

experiment example 1

[0038] Breeding test: 4,000 pairs of healthy breeding pigeons (500 pairs in each warehouse) were selected from the eight warehouses in the meat pigeon breeding farm for the test. Among them, seven pigeons in the warehouse are the experimental group (group I), and the eight pigeons in the warehouse are the control group (group II). The feeding conditions are caged, and the feeding rations, medicines, drinking water, health sand and other management conditions are the same. Breeding pigeon eggs are hatched by the hatching house, and 0-day-old healthy pigeons are brought back from the hatching house and fed by litters of breeding pigeons, each with 4 pigeons. Group I was fed with conventional diets; group II was on the basis of conventional diets, with the addition of 5% of the total weight of the poultry feed prepared in Example 2; Feeding and drinking, the light is sunshine, the mental state and feeding situation of pigeons were observed and recorded every day, and the pigeons...

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Abstract

The invention discloses a preparation method and application of a compound probiotic feed for poultry. The compound probiotic feed for poultry includes Lactobacillus bulgaricus P-3 strain, the taxonomic name is Lactobacillus bulgaricus, and is preserved in the Guangdong Microbial Strain Preservation Center. The number is GDMCC No: 60252, and the preservation address is 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City, and the preservation date is October 20, 2017. The Lactobacillus bulgaricus P-3 strain of the present invention can be used as a feed additive and added to poultry feed, which can promote the growth of poultry, improve the nutritional level of poultry, increase feed remuneration, reduce the ratio of feed to meat, improve breeding benefits, and inhibit pathogenic bacteria It can reduce the use of antibiotics in poultry farming, improve the immune function and anti-stress ability of poultry, reduce mortality, and realize safe, effective and green production of poultry products. It has a good application prospect and promotes the healthy and stable development of poultry farming. Market value.

Description

technical field [0001] The invention belongs to the technical field of poultry breeding, and more specifically, the invention relates to Lactobacillus bulgaricus P-3 strain and its application in preparing poultry compound probiotic feed. Background technique [0002] With the increasing demand for poultry (meat pigeons) in domestic and foreign markets, my country's poultry industry is rapidly developing towards scale, product commercialization and management enterprise. At present, there are more than 30 million pairs of breeding pigeons in the country, with an annual output of about 620 million pigeons, and the annual growth rate is 10%-15%. At the same time, the occurrence and epidemic of poultry diseases are complicated, and the food safety problems caused by the irrational use of drugs in production have attracted people's attention. SUMMARY OF THE INVENTION [0003] The object of the present invention is to: overcome the problems such as the abuse of antibiotics exi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K50/75A23K50/70A23K10/18C12R1/225
CPCA23K10/18A23K50/70A23K50/75C12N1/205C12R2001/225
Inventor 邹永新李殿鑫黄笑筠肖智远冯开容郑秀容李剑杰姜文联张君李莉娜赖秋英康艳梅梁日勤
Owner 广东科贸职业学院
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