Building method of human spongioblastoma cell SCID mouse brain transplantation tumor model

A technology of malignant glioma and construction method, which is applied in the field of construction of human glioblastoma cell SCID mouse brain transplantation tumor model, and achieves the effect of significant brain edema and rapid tumor growth

Inactive Publication Date: 2018-06-22
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In view of this, the present invention aims at the lack of HCMV-infected human glioma animal models similar to the natural

Method used

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  • Building method of human spongioblastoma cell SCID mouse brain transplantation tumor model
  • Building method of human spongioblastoma cell SCID mouse brain transplantation tumor model
  • Building method of human spongioblastoma cell SCID mouse brain transplantation tumor model

Examples

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Embodiment 1

[0022] Example 1 Establishment of human malignant glioma cell SCID mouse brain xenograft model

[0023] Include the following steps:

[0024] Step 1. Resuscitate malignant glioma cells frozen in liquid nitrogen tank U871×10 7 cells / 100μl, 100μl / only, at 37℃5%CO 2 Cultivate overnight in an incubator with 10% high glucose DMEM / F12 (Hyclone), add 5 μL of Hoechst33342 to label primary malignant glioma cells, continue culturing for 48 hours, and collect cells to prepare primary malignant glioma cells;

[0025] Step 2. Select male SCID mice, weighing 210-110g, and divide them into 5 groups: ① Inoculate primary malignant glioma cells into the brain; ② Inoculate primary malignant glioma cells into the brain and infect HCMV; ③ Inoculate the brain Primary malignant glioma cells from primary culture infected with HCMV and treated with USP18 high-expression adenoviral vector; ④ Inoculated in the brain with primary malignant glioma cells from primary culture infected with HCMV and treate...

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Abstract

The invention discloses a building method of a human spongioblastoma cell SCID mouse brain transplantation tumor model. The method comprises the following steps of preparing primary spongioblastoma cells; selecting male SCID mice; dividing the mice into five groups (the first group of mice are inoculated with primary generation spongioblastoma cells in the brain; the second group of mice are inoculated with primary generation spongioblastoma cells in the brain and are infected with HCMV; the third group of mice are inoculated with primary spongioblastoma cells, and are infected with HCMV, andUSP18 high expression adenovirus carriers are used for processing the group; the fourth group of mice are inoculated with primary generation spongioblastoma cells in the brain and are infected with HCMV, interference adenovirus carrier USP18 small interference adenovirus carriers USP18 are used for treating the group of mice; the fifth group of mice is a normal control group); determining a targeted point; performing aseptic inoculation of single-cell suspension; during experiment termination, cutting the head; taking the brain; identifying whether the building of the human spongioblastoma cell SCID mouse brain transplantation tumor model is successful or not. The tumor model can better embody the practical infection state of the HCMV infected human spongioblastoma.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a method for constructing a human malignant glioma cell SCID mouse brain transplanted tumor model. Background technique [0002] Previous studies have found that the expression of HCMV gene expression products is significantly increased in malignant gliomas, and is positively correlated with the degree of tumor malignancy. Malignant gliomas are not sensitive to surgery, radiotherapy, and chemotherapy, and it is urgent to seek targeted treatments. HCMV-infected cells participate in anti-virus and anti-tumor effects through NF-КB and TBK1-IRF3 type I interferon pathways, and a variety of ubiquitin-specific proteases act on different targets of this pathway for negative regulation. In previous studies, we found that HCMV infected astrocytes for 6 hours, and the molecules whose gene expression levels changed more than 10 times were all interferon-stimul...

Claims

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Application Information

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IPC IPC(8): A61K35/13A61D7/00A01K67/027
CPCA01K67/0271A61D7/00A61K35/13
Inventor 张丽王斌于红李玲钱冬萌胡明王爽秦子英赵舒畅吴文迪
Owner QINGDAO UNIV
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