Building method of human spongioblastoma cell SCID mouse brain transplantation tumor model

Abstract

The invention discloses a building method of a human spongioblastoma cell SCID mouse brain transplantation tumor model. The method comprises the following steps of preparing primary spongioblastoma cells; selecting male SCID mice; dividing the mice into five groups (the first group of mice are inoculated with primary generation spongioblastoma cells in the brain; the second group of mice are inoculated with primary generation spongioblastoma cells in the brain and are infected with HCMV; the third group of mice are inoculated with primary spongioblastoma cells, and are infected with HCMV, andUSP18 high expression adenovirus carriers are used for processing the group; the fourth group of mice are inoculated with primary generation spongioblastoma cells in the brain and are infected with HCMV, interference adenovirus carrier USP18 small interference adenovirus carriers USP18 are used for treating the group of mice; the fifth group of mice is a normal control group); determining a targeted point; performing aseptic inoculation of single-cell suspension; during experiment termination, cutting the head; taking the brain; identifying whether the building of the human spongioblastoma cell SCID mouse brain transplantation tumor model is successful or not. The tumor model can better embody the practical infection state of the HCMV infected human spongioblastoma.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a method for constructing a human malignant glioma cell SCID mouse brain transplanted tumor model. Background technique [0002] Previous studies have found that the expression of HCMV gene expression products is significantly increased in malignant gliomas, and is positively correlated with the degree of tumor malignancy. Malignant gliomas are not sensitive to surgery, radiotherapy, and chemotherapy, and it is urgent to seek targeted treatments. HCMV-infected cells participate in anti-virus and anti-tumor effects through NF-КB and TBK1-IRF3 type I interferon pathways, and a variety of ubiquitin-specific proteases act on different targets of this pathway for negative regulation. In previous studies, we found that HCMV infected astrocytes for 6 hours, and the molecules whose gene expression levels changed more than 10 times were all interferon-stimul...

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Problems solved by technology

[0004] In view of this, the present invention aims at the lack of HCMV-infected human glioma animal models similar to the natural infection mode at present, and provides a method for constructing a human malignant glioma cell SCID mouse brain xenograft model

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