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Application of amlexanox to inhibition of epithelial-mesenchymal transition and anti-tumor metastasis

A technology for anti-tumor metastasis and amlezanox, which is applied in the field of anti-epithelial-mesenchymal transition and anti-tumor metastasis of amlezanox, can solve the problems of severe infection, low immunity, metabolic disorders, etc., and achieve the inhibition of epithelial-mesenchymal transition and tumor metastasis, huge socio-economic benefits

Active Publication Date: 2018-07-06
LEADINGEN BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The low immunity of tumor patients leads to secondary severe infect

Method used

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  • Application of amlexanox to inhibition of epithelial-mesenchymal transition and anti-tumor metastasis
  • Application of amlexanox to inhibition of epithelial-mesenchymal transition and anti-tumor metastasis
  • Application of amlexanox to inhibition of epithelial-mesenchymal transition and anti-tumor metastasis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Experimental materials and equipment: PC3 cell line ( CRL-1435 TM ), (Cat no114-07), double chemiluminescent reporter system (pLenti-EcadPm-mCherry-PA-Spacer-VimPm-eGFP-PA-hPKG-Puro), small molecule compound library (Taosu)

[0036] Experimental steps:

[0037] 1. Transfect the double chemiluminescence system and verify the working efficiency

[0038] 1.1 Inoculate the single cell suspension of the human prostate cancer cell line PC3 evenly into a six-well plate, and when the cells grow to a density of about 80%, use (Cat no114-07) transfection reagent will double fluorescent reporter system pLenti-EcadPm-mCherry-PA-Spacer-VimPm-eGFP-PA-hPKG-Puro( figure 1 ) into PC3 cells, the specific transfection method refers to Transfection Reagent Instructions.

[0039] 1.2 After 72 hours of transfection, observe the cell state under a microscope. When the cell state is good, add 1ug / ml puromycin to the medium for cell selection.

[0040] 1.3 After 48 hours of puromyci...

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Abstract

The invention provides application of amlexanox to inhibition of epithelial-mesenchymal transition (EMT) and anti-tumor metastasis. The effective concentration of the amlexanox for inhibition of epithelial-mesenchymal transition and anti-tumor metastasis is 100 nM to 10 muM. Whether various micromolecular compounds are EMT target points or not is screened by an efficient and dynamic screening method, the obtained amlexanox can effectively inhibit epithelial-mesenchymal transition and tumor metastasis, a new method is provided for treatment target points for treating tumor metastasis clinically, and great social and economic benefits can be brought.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the use of amlezanox in inhibiting epithelial-mesenchymal transition and anti-tumor metastasis. Background technique [0002] With the rapid development of the global economy, science and humanities, human beings are paying more and more attention to the topic of global health. According to the 2015 CBD 【1】 Statistical analysis of the global burden of disease shows that ischemic heart disease, cerebrovascular disease and other diseases threaten human health, among which lung cancer, liver cancer, colon cancer, breast cancer and other cancers also top the list, becoming one of the number one killers of human beings . From the clear definition of cancer to the present, whether government agencies, researchers or civil organizations have invested decades in order to uncover the mystery of cancer and overcome cancer, but until recently, with economic development, population increa...

Claims

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Application Information

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IPC IPC(8): A61K31/436A61P35/04
CPCA61K31/436
Inventor 高维强朱鹤程姹萍吉忠忠
Owner LEADINGEN BIOTECH INC
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