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Drug against renal fibrosis and preparation method thereof

A renal fibrosis and drug technology, which is applied in the field of drugs for treating renal fibrosis and their preparation, can solve the problems of poor specificity and poor efficacy of western medicine, and achieves the effect of inhibiting transcription level and inhibiting epithelial-mesenchymal transition. Effect

Active Publication Date: 2021-11-05
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the specificity of western medicine for anti-renal fibrosis is not strong, and the curative effect is not good.
The traditional Chinese medicine pathogenesis of renal fibrosis is deficiency in origin and excess in superficiality. The deficiency in origin is mainly the deficiency of kidney qi, and the excess in superficiality mainly refers to blood stasis, damp heat, and flooding. Traditional Chinese medicine has unique clinical advantages in treating renal fibrosis, but it is still No specific traditional Chinese medicine composition can effectively treat renal fibrosis

Method used

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  • Drug against renal fibrosis and preparation method thereof
  • Drug against renal fibrosis and preparation method thereof
  • Drug against renal fibrosis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Based on high-throughput sequencing technology, the regulation of 35 kinds of traditional Chinese medicine ethanol extracts on five key genes of renal fibrosis in human renal tubular epithelial cells HK2 was detected.

[0076] 1. Preparation of single herbal extracts of 35 kinds of Chinese herbal medicines:

[0077] Atractylodes macrophylla, puffball, spider plant, cicada slough, snake slough, shegan, peach branch, pterygium, duckweed, Cimicifuga, white jelly, sinew grass, saponin thorn, neem bark, light bamboo leaf, Tongcao, Changshan, red flower dragon Gallbladder, Xu Changqing, Ligustrum lucidum, Sijiqing, Spatholobus yunnanensis, Nuxychia chinensis, lotus root, three needles, Shannai, Eucommia, loquat leaves, cnidium, licorice, Eucommia leaves, astragalus, ginseng, motherwort, peony Skin.

[0078] Take the above Chinese medicinal materials, crush them into 80-100 mesh powder, add 15 times the volume of 90% ethanol, standard Soxhlet reflux extraction for 3 hours, co...

Embodiment 2

[0086] Take the composition of Atractylodes macrocephala 2.5g, puffball 2.5g, spider plant 2.5g, and cicada slough 2.5g as examples to verify the efficacy of the traditional Chinese medicine composition:

[0087] 1. Extraction of the composition:

[0088] Crush the formula and weigh it as 80-100 mesh powder, weigh it according to the proportion, mix it, add 15 times the volume of 90% ethanol, standard Soxhlet reflux extraction for 3 hours, concentrate the extract for 20 minutes to a volume of 2 mL, and use a lyophilizer to lyophilize under reduced pressure After 24 hours, the extract was made into freeze-dried powder, and a certain quality of the freeze-dried powder was weighed, dissolved in DMSO to make the mother liquor of the extract, and stored at -20°C.

[0089] 2. Regulation of the extract on epithelial-mesenchymal transition of human renal tubular cells

[0090] 2.1 HK2 cells were plated in 12-well plates at 80,000 / well. After overnight, serum was starved for 24 hours,...

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Abstract

The invention relates to the technical field of traditional Chinese medicine, in particular to a medicine for treating renal fibrosis and a preparation method thereof. The 31 kinds of Chinese medicinal materials provided by the present invention all have the effect of anti-renal fibrosis, anti-cardiorenal syndrome, anti-heart failure, anti-nephrotic syndrome and / or anti-renal failure drugs. The composition composed of cicada slough can inhibit the epithelial-mesenchymal transition of renal tubular cells and the transcription level of key genes, so it can effectively treat renal fibrosis, cardiorenal syndrome, heart failure, nephrotic syndrome, and renal failure.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a medicine for treating renal fibrosis and a preparation method thereof. Background technique [0002] Renal fibrosis is the common pathological basis for the development of various renal diseases to end-stage renal failure, and is an important pathological process of cardiorenal syndrome and heart failure. The development of new therapies for renal fibrosis is of great significance for renal failure and cardiorenal syndrome. Renal fibrosis is associated with dysregulated expression of various genes, including human plasminogen activator inhibitor 1 gene (SERPINE1), type Ⅰ collagen α1 gene (COL1A1), human interleukin 13 receptor α1 (IL13RA1), endothelin receptor B(EDNRB) and SMAD4 are key genes affecting renal fibers. Inhibiting the key genes of renal fibrosis can effectively inhibit the pathological process of renal fibrosis, prevent the accumulation of ex...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/906A61K36/899A61K36/898A61K36/88A61K36/284A61P13/12A61K35/583A61K35/64
CPCA61K35/583A61K35/64A61K36/07A61K36/185A61K36/19A61K36/234A61K36/25A61K36/27A61K36/284A61K36/29A61K36/46A61K36/483A61K36/484A61K36/486A61K36/515A61K36/53A61K36/56A61K36/58A61K36/62A61K36/638A61K36/704A61K36/71A61K36/73A61K36/736A61K36/80A61K36/88A61K36/898A61K36/899A61K36/906A61K2236/333A61K2236/51A61P13/12A61K2300/00
Inventor 乔连生谢兰张晓玲郭弘妍王栋邢婉丽程京
Owner CAPITALBIO CORP
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