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Application of fangchinoline in preparation of drugs for inhibiting metastasis of lung cancer

A technology for fangchinoline and lung cancer metastasis, applied in the field of medicine, can solve problems such as tetrandrine that no research shows, and achieve the effects of inhibiting metastasis and improving the quality of life

Inactive Publication Date: 2019-11-01
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, whether in terms of mechanism or drug use, lung cancer metastasis is quite different from the proliferation and growth of lung cancer. At present, there is no research to show whether fangchinoline can inhibit the metastasis of lung cancer cells.

Method used

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  • Application of fangchinoline in preparation of drugs for inhibiting metastasis of lung cancer
  • Application of fangchinoline in preparation of drugs for inhibiting metastasis of lung cancer
  • Application of fangchinoline in preparation of drugs for inhibiting metastasis of lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Fangchinoline inhibits the proliferation activity of lung cancer cells

[0035] 1. Experimental method

[0036] (1) Cell culture

[0037] Lung cancer cells were cultured in DMEM high-glucose medium with 10% fetal bovine serum at 37°C, 5% CO 2 Cultured in an incubator, passaged once every 24-48 hours.

[0038] (2) CCK8 experiment

[0039] Lung cancer NCI-H1299 cells in the logarithmic growth phase were inoculated on a 96-well plate at a cell density of 10,000 cells per well. After culturing for 24 hours, they were divided into groups. Each group had 6 replicate wells. Each group was treated as follows: blank group (i.e. adjusted zero hole), the control group (DMEM medium with 10% fetal bovine serum), the intervention groups of fangchinoline at each dose (adding fangchinoline 2.5 μM, 5 μM, 10 μM, 20 μM, 25 μM, 30 μM, 35 μM, 40 μM 10 % fetal bovine serum in DMEM medium). After culturing for 24h and 48h, add 10 μL of CCK8 solution to each well, incubate for 2...

Embodiment 2

[0042] Example 2 Fangchinoline inhibits the migration ability of lung cancer cells

[0043] 1. Experimental method

[0044] Using scratch test, the concentration of NCI-H1299 cells was 1×10 5 Inoculate in a 6-well plate and culture. After the cells adhere to the wall, use a 100 μL pipette tip to scratch the bottom of the culture plate with the word "one", wash with PBS, and replace with new medium. Set up the control group and the experimental group respectively. The experimental group was cultured in DMEM medium with 10% fetal bovine serum, and the experimental group was intervened with DMEM medium with 10% fetal bovine serum containing 1.25 μM, 2.5 μM, and 5 μM fangchinoline. Each group was set up with 3 replicates. Wells were cultured in an incubator, and after 0h, 24h and 48h, cell migration results were observed and photographed.

[0045] 2. Experimental results

[0046] Fangchinoline inhibits the migration ability of NCI-H1299 cells image 3 As shown, the effect of f...

Embodiment 3

[0047] Example 3 Fangchinoline inhibits the invasion ability of lung cancer cells

[0048] 1. Experimental method

[0049] Using the Transwell experiment, add 600 μL of medium containing 15% FBS to the lower chamber of the Transwell chamber. NCI-H1299 cells were digested and resuspended in serum-free medium. After counting, a cell suspension with twice the target concentration was prepared, and a fangchinoline solution with twice the target concentration was prepared. The control group and the experimental group were set up. Three replicate wells were set up in each group, and the cell suspension was mixed with the same volume of fangchinoline alkali solution of each group respectively, so that the final concentration of the experimental group was 1.25 μM, 2.5 μM, 5 μM, and 200 μL per well, 3×10 4 cells were seeded in the upper chamber. Place them in an incubator for 24 hours, add PBS to the upper and lower chambers to wash three times, and fix with 4% paraformaldehyde for 1...

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Abstract

The present invention discloses an application of fangchinoline in preparation of drugs for inhibiting metastasis of lung cancer. Specifically, the fangchinoline can significantly inhibit epithelial-mesenchymal transition of lung cancer cells, at the same time has a significant inhibitory effect on the lung cancer cells, and can effectively inhibit migration ability and invasion ability of the lung cancer cells. Therefore, the fangchinoline can significantly inhibit further deterioration of the lung cancer, significantly inhibit the metastasis of the lung cancer, and improve survival rate andlife quality of patients. In addition, the fangchinoline is an effective ingredient in traditional Chinese medicinal materials of rhizoma sinomeni cauliset, etc., and wide in sources, safe and low intoxicity. Therefore, the fangchinoline is used in treatment of the lung cancer, inhibits the metastasis of the lung cancer cells in the patients with the lung cancer, is remarkable in effects, also small in dosage and small in toxic and side effects, can greatly improve the life quality of the patients, and has a broad development space and a good application prospect in the preparation of the drugs for inhibiting the metastasis of the lung cancer cells.

Description

technical field [0001] The invention belongs to the technical field of medicine. More specifically, it relates to the application of fangchinoline base in the preparation of drugs for inhibiting lung cancer metastasis. Background technique [0002] Lung cancer is the most common cancer in the world, and its morbidity and mortality rank first among malignant tumors, posing a great threat to human health and life. In my country, the incidence of lung cancer ranks first among men and second among women. In recent years, the incidence rate in my country has been increasing. The World Health Organization predicts that by 2025, China will have more than 1 million new cases of lung cancer each year, making it the largest lung cancer country in the world. To make matters worse, most patients were diagnosed with lung cancer in the middle and late stages, and lost the best time for surgical treatment; even though some patients underwent early surgical treatment, the recurrence rate ...

Claims

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Application Information

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IPC IPC(8): A61K31/4748A61P35/04
CPCA61K31/4748A61P35/04
Inventor 肖建勇陈博南
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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