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Application of mandarin orange oil droplet layer crude extract in inhibiting metastasis of ovary carcinoma

An oil cell layer and crude extract technology, which is applied to the application field of the crude extract of Ou citrus oil cell layer in inhibiting the metastasis of ovarian cancer, and can solve the problems of no report on inhibiting tumor metastasis and little pharmaceutical activity.

Inactive Publication Date: 2014-07-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The oil cell layer crude extract, a by-product of Ou mandarin orange processing, has relatively few reports on its pharmaceutical activity, and most of them focus on the activities of lowering blood pressure, lowering blood sugar, anti-oxidation, and enhancing immune function, but its inhibitory effect on tumor metastasis has not been reported. See the report

Method used

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  • Application of mandarin orange oil droplet layer crude extract in inhibiting metastasis of ovary carcinoma
  • Application of mandarin orange oil droplet layer crude extract in inhibiting metastasis of ovary carcinoma
  • Application of mandarin orange oil droplet layer crude extract in inhibiting metastasis of ovary carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In the present invention, the source of the oil cell layer crude extract of Ou mandarin orange is obtained from the oil cell layer of citrus plant Ou mandarin, and its preparation method is carried out according to the following two steps:

[0025] (1) Weigh 1 g of Ou mandarin oil cell layer freeze-dried powder, and use 20 mL of 80% acetone (solid-to-liquid ratio 1:20) to sonicate for 30 min at a frequency of 60 kHz and a power of 30 W. The extract was centrifuged at 4000 rpm for 10 min on a centrifuge, and this was repeated twice. The supernatant was combined, evaporated to dryness on a rotary evaporator, and dissolved in 10 mL of deionized water. The extraction process of the crude oil cell layer extract of Ou mandarin figure 1 .

[0026] (2) The water extract was purified with a solid phase extraction SPE C18 column (Waters, 12 cc, 2 g). The purification process was divided into five steps: activation, equilibration, sample loading, rinsing and elution: the SPE ca...

Embodiment 2

[0028] The crude extract of oil cell layer of Ou mandarin orange extracted in Example 1 was tested for inhibition of proliferation of human ovarian cancer cells, and it was found that the crude extract of oil cell layer of Ou mandarin orange did not affect the proliferation ability of cells within a certain concentration range. The experimental methods and results are as follows:

[0029] The crude extract of Ou mandarin oil cell layer acts on human ovarian cancer cells (SKOV3 cells) to inhibit cell proliferation after 24 h. SKOV3 cells were cultured in 90% RPMI-1640 (purchased from Gibco, Grand Island, NY, US) and 10% fetal bovine serum (Gibco, Carlsbad, CA, US). The specific steps are as follows: After digesting the cells in the logarithmic growth phase, pipette them into a single cell suspension and inoculate them in a 96-well culture plate; 5 × 10 3Cells / well, 200 microliters of culture medium per well, cultured overnight in a 37°C, 5% CO2 incubator; adding crude extracts...

Embodiment 3

[0032] The crude extract of the oil cell layer of Ou mandarin orange extracted in Example 1 was used to study the anti-metastasis activity of human ovarian cancer, and it was found that the crude extract of the oil cell layer of Ou mandarin orange can inhibit the scratch repair ability of tumor cells, and the distance between the scratches is higher than that of significantly increased in the control group. The experimental methods and results are as follows:

[0033] Human ovarian cancer cell SKOV3 was planted in a 24-well plate, cultured with complete medium until the cells were almost confluent, and after forming a monolayer of cells, starved overnight. Make a scratch injury on the cell surface of the monolayer with a 100 µL pipette tip. Then wash the scratches with culture medium, culture cells with complete medium, and set up the following groups: control group without serum, 4 μg / mL group without serum, 20 μg / mL group without serum, control group with serum , added to ...

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Abstract

The invention provides application of a mandarin orange oil droplet layer crude extract in preparation of medicaments and health products for preventing and treating metastasis of ovary carcinoma. Researches indicate that a natural product with the effect of inhibiting tumor metastasis can be extracted from the oil droplet layer of mandarin orange, has the activity of inhibiting cell migration of a human ovarian carcinoma cell line SKOV3, and has a definite effect of inhibiting tumor metastasis. In the application, an oil droplet layer crude extract can be extracted from the mandarin orange in citrus to perform researches on inhibiting bioactivity of tumor metastasis; research results confirm that the oil droplet layer crude extract can be used for remarkably reducing the moving ability of the human ovarian carcinoma cell line SKOV3 and can inhibiting occurrence of epithelial-mesenchymal transition induced by TGF-beta (transforming growth factor-beta), and prompt that the oil droplet layer crude extract has potential activity in inhibiting tumor metastasis. Health products prepared from the mandarin orange oil droplet layer crude extract and other effective components which can be monomer or mixture, as well as permissible food additives can be used for preventing and treating occurrence of tumor metastasis.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of the oil cell layer crude extract of natural sources in the prevention and treatment of ovarian cancer and other related cancers, in particular to the application of the oil cell layer crude extract of Ou mandarin orange in the preparation of anti-cancer drugs and functional foods or health products in the application. Background technique [0002] With the changes of many natural and social factors such as human living environment, living pressure, population aging, etc., the incidence and mortality of malignant tumors continue to rise, seriously threatening human health and life. Tumor metastasis is an important cause of patient death, and 90% of tumor patients die of recurrence and metastasis within five years. Ovarian cancer is one of the three major malignant tumors of the female reproductive system. Its incidence rate is second only to cervical cancer and endometria...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A23L1/30A61P35/04
Inventor 朱虹陈昆松何俏军杨波李鲜孙崇德常琳琳
Owner ZHEJIANG UNIV
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