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Influenza virus A IgA antibody immunofluorescence detection test strip, and preparation method, detection method, and applications thereof

A type of influenza A virus, immunofluorescence detection technology, applied in the field of medical testing, can solve problems such as high price, inability to promote at the grassroots level, and missed detection.

Active Publication Date: 2018-07-06
GUANGZHOU RHFAY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since this detection technology requires an expensive PCR detector and a certified laboratory with certain detection conditions, it cannot be promoted at the grassroots level
Antigen colloidal gold immunization technology directly collects throat swabs of patients, and can judge the patient's infection with influenza virus by naked eyes; but it has the disadvantages of low sensitivity and easy to cause missed detection

Method used

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  • Influenza virus A IgA antibody immunofluorescence detection test strip, and preparation method, detection method, and applications thereof
  • Influenza virus A IgA antibody immunofluorescence detection test strip, and preparation method, detection method, and applications thereof
  • Influenza virus A IgA antibody immunofluorescence detection test strip, and preparation method, detection method, and applications thereof

Examples

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Embodiment 1

[0060] Example 1: A kind of influenza A virus IgA antibody immunofluorescence detection test strip and preparation method thereof (capture method)

[0061] Such as figure 1 As shown, the influenza A virus IgA antibody immunofluorescence detection test strip of this embodiment includes: a back plate 5 , a sample pad 1 , a marker pad 2 , a nitrocellulose membrane 3 and an absorbent paper 4 . Among them, the absorbent paper 4, the nitrocellulose membrane 3, the marking pad 2, and the sample pad 1 are adhered on the back plate 5 in sequence.

[0062] The nitrocellulose membrane 3 includes the detection line T of the influenza A virus NP protein antigen polypeptide fragment and the control line C of the human IgA antibody. The amino acid sequence of the influenza A virus NP protein antigen polypeptide fragment is shown in the sequence table Seq ID NO.1.

[0063] Marker pad 2 is provided with anti-human IgA antibody-fluorescent microsphere markers. The anti-human IgA antibody i...

Embodiment 2

[0076] Example 2: Preparation of another type of influenza A virus IgA antibody immunofluorescence test strip (indirect method)

[0077] Such as figure 1 As shown, the influenza A virus IgA antibody immunofluorescence detection test strip of this embodiment includes: a back plate 5 , a sample pad 1 , a marker pad 2 , a nitrocellulose membrane 3 and an absorbent paper 4 . Among them, the absorbent paper 4, the nitrocellulose membrane 3, the marking pad 2, and the sample pad 1 are adhered on the back plate 5 in sequence.

[0078] Nitrocellulose membrane 3 includes a test line T for anti-human IgA antibody and a control line C for antibody to influenza A virus NP protein. The anti-human IgA antibody is selected from goat anti-human IgA polyclonal antibody, mouse anti-human IgA monoclonal antibody, rabbit anti-human IgA monoclonal antibody or rabbit anti-human IgA polyclonal antibody. Influenza A virus NP protein antibody is selected from mouse anti-influenza A virus NP protei...

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Abstract

The invention provides an influenza virus A IgA antibody immunofluorescence detection test strip, and a preparation method thereof. The influenza virus A IgA antibody immunofluorescence detection teststrip comprises a sample pad, a label pad, a cellulose nitrate membrane, a water absorbent paper, and a backboard; the cellulose nitrate membrane comprises a detection line of influenza virus A NP protein antigen polypeptide fragments, and a human IgA antibody control line; the label pad is provided with anti-human IgA antibody-fluorescence microsphere markers. The influenza virus A IgA antibodyimmunofluorescence detection test strip is extremely high in detection sensitivity; expensive PCR detector is not needed; detection results can be observed with naked eyes; rapid screening on influenza virus A is realized. The invention also provides a detection method and applications of the influenza virus A IgA antibody immunofluorescence detection test strip. The detection method and the stepsare simple; operation is convenient; results are obtained quickly; and the influenza virus A IgA antibody immunofluorescence detection test strip is worthy of wide popularization.

Description

technical field [0001] The invention belongs to the field of medical inspection, and relates to a test strip for detecting influenza virus, in particular to a test strip for immunofluorescence detection of IgA antibody of influenza A virus, a preparation method, a detection method and application thereof. Background technique [0002] Influenza virus is a virus that can infect any age group and can infect the whole world, with great influence and destructive power. Influenza viruses can be divided into influenza A, influenza B, and influenza C, among which influenza A virus is the most contagious and harmful. According to the difference of hemagglutinin (H protein) and neuraminidase (N protein) on the surface of influenza A virus, H can be divided into 17 subtypes (H1~H17), and N has 10 subtypes (N1~N10). . Seasonal influenza is an acute viral infection caused by influenza virus. Common influenza A viruses that cause seasonal influenza in mainland China are subtypes H1N1 a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/533
CPCG01N33/533G01N33/56983G01N2333/11
Inventor 游爱萍李有生刘辉刘轶锴
Owner GUANGZHOU RHFAY BIOTECH CO LTD
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