A kind of fermentation process of L-tryptophan
A fermentation process, tryptophan technology, applied in the field of L-tryptophan fermentation technology, can solve the problems of complex cultivation process and difficult control of parameters, and achieve the goal of improving acid production efficiency, less residual sugar, and promoting utilization and translocation Effect
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Embodiment 1
[0029] A fermentation process for L-tryptophan, comprising the steps of:
[0030] Escherichia coli CCTCC M 2011316 was cultured to a concentration of 1 × 10 7 cfu / mL seed solution, and then inoculate the fermentation medium (glucose 20g / L, corn steep liquor 10g / L, ammonium sulfate 5g / L, dipotassium hydrogen phosphate 2g / L, phosphoric acid Potassium dihydrogen 2g / L, magnesium sulfate 1.5g / L, citric acid 1.5g / L, ferrous sulfate 70mg / L, sodium sulfate 20mg / L, manganese sulfate 7mg / L, zinc sulfate 7mg / L, cobalt chloride 6mg / L, copper sulfate 0.9mg / L), the fermentation temperature is controlled at 36°C, the dissolved oxygen is controlled at 20%, the tank pressure is 0.05MPa, and the residual sugar is controlled at not less than 1.0 by adding a glucose solution with a concentration of 100g / L. %, the pH is controlled at 6.8 by feeding ammonia water; fermentation is stopped at 30h to obtain L-tryptophan fermentation broth;
[0031] Utilize high-speed disc separator to centrifuge L-...
Embodiment 2
[0034] A fermentation process for L-tryptophan, comprising the steps of:
[0035] The tnaA, trpR and tyrR gene knockout or inactivated Escherichia coli ATCC 27325 were cultivated to a concentration of 1×10 7 cfu / mL seed solution, and then inoculate the fermentation medium (glucose 20g / L, corn steep liquor 10g / L, ammonium sulfate 5g / L, dipotassium hydrogen phosphate 2g / L, phosphoric acid Potassium dihydrogen 2g / L, magnesium sulfate 1.5g / L, citric acid 1.5g / L, ferrous sulfate 70mg / L, sodium sulfate 20mg / L, manganese sulfate 7mg / L, zinc sulfate 7mg / L, cobalt chloride 6mg / L, copper sulfate 0.9mg / L), the fermentation temperature is controlled at 36°C, the dissolved oxygen is controlled at 20%, the tank pressure is 0.05MPa, and the residual sugar is controlled at not less than 1.0 by adding a glucose solution with a concentration of 100g / L. %, the pH is controlled at 7 by feeding ammonia water; fermentation is stopped at 30h to obtain L-tryptophan fermentation broth;
[0036] Uti...
Embodiment 3
[0039] The influence of various factors on the production of L-tryptophan:
[0040] Taking Example 1 as the test group, the fermentation medium was set to 100L, and the L-tryptophan yield in the upper layer feed liquid and the filtrate was detected, and the detection method was determined by HPLC, with specific reference to the British Pharmacopoeia of 2007 editions;
[0041] A control group is set, wherein, control group 1: control group 1 is: only dialysis culture treatment is performed, tourmaline powder and heat treatment are not used, and the rest are the same as in Example 1; control group 2: after the fermentation is completed, tourmaline powder and dialysis culture treatment are performed, No heat treatment, the rest are the same as in Example 1; Control group 3: after the fermentation is completed, heat treatment and dialysis culture treatment are performed, and tourmaline powder treatment is not used, and the rest are the same as in Example 1; Detect the content of L-...
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