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Fluorescent quantitative PCR (polymerase chain reaction) primer for detecting coxsackie virus, probe, kit and method

A Coxsackie virus, fluorescence quantitative technology, applied in the field of kits, probes, and fluorescent quantitative PCR primers for detecting Coxsackie virus, can solve problems such as low sensitivity and difficult early diagnosis, and achieve sensitive, accurate, and specific detection. Good performance and good primer specificity

Inactive Publication Date: 2018-07-24
东莞市第八人民医院 +1
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  • Abstract
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Problems solved by technology

Currently commonly used virological laboratory diagnostic methods include immunological methods, virus isol

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  • Fluorescent quantitative PCR (polymerase chain reaction) primer for detecting coxsackie virus, probe, kit and method

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Embodiment Construction

[0046] For the convenience of those skilled in the art to understand, below in conjunction with embodiment and appended figure 1 To further describe the present invention, the content mentioned in the embodiment is only used to illustrate the present invention and is not intended to limit the scope of protection claimed by the present invention.

[0047] Concrete experimental conditions and methods are not indicated in the following examples, usually according to conventional conditions such as: editors such as J. Sambrook, Science Press, 1992, Molecular Cloning Experiment Guide (Second Edition): D.L. Spector etc., Science Publishing House, 2001, Cell Experiment Guide: Lu Hongsheng, Science Press, 1982, or according to the conditions suggested by the manufacturer.

[0048] A fluorescent quantitative PCR primer for detecting Coxsackie virus, the sequence of the primer is as follows:

[0049] Coxsackievirus A6-VP1F: 5'-TTCGGGTGTACATGAGAATTAAG-3'

[0050] Coxsackievirus A6-VP1R...

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Abstract

The invention relates to the technical field of biological detection, in particular to a fluorescent quantitative PCR (polymerase chain reaction) primer for detecting coxsackie virus, a probe, a kit and a method. A sequence of the primer is VP1F: 5'-TTCGGGTGTACATGAGAATTAAG-3'; VP1R: 5'-CGCCTTCATAATCCGTGGTGGTT-3'; a sequence of the probe is VP1P: 5'-TTGGGTACCTAGACCCCTTC-3'. The kit comprises the primer and the probe. The kit has the advantages that the existence of an amplified product is indicated by the specific combination of the probe of a fluorescent marker and the virus target sequence, and the existence of the coxsackie virus is detected; the size of copy number of virus is calculated by contrasting with gradient standard products, the accuracy is good, and the sensitivity is high.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a fluorescent quantitative PCR primer, probe, kit and method for detecting Coxsackie virus. Background technique [0002] Hand, foot and mouth disease (HFMD) is an important infectious disease that seriously threatens the health of children. In the past, the main pathogens that caused HFMD were Coxsackievirus A16 (CA16) and Enterovirus 71 (EV71). Virus type A6 (CA6) has become the main circulating strain. The HFMD epidemic caused by CA6 occurred in Osaka, Japan in 2011, 2013, and 2015; the main pathogen of the HFMD epidemic in France from 2014 to 2015 was CA6; in 2015, CA6 caused the military camp in Texas, USA Outbreaks of hand, foot and mouth disease in China. In recent years, Chinese scholars have also strengthened the monitoring of non-CA16 and non-EV71 enteroviruses, and found that CA6 has become the main epidemic strain in most parts of the country. In Donggu...

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11C12R1/93
CPCC12Q1/6851C12Q1/701C12Q2531/113C12Q2563/107
Inventor 钟柏茂陆小梅彭琪黎四平蒋再学李文瑞
Owner 东莞市第八人民医院
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