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A method for rapid extraction and detection of geranylgeranyl pyrophosphate in plant tissues

A plant tissue and mixture technology, applied in the field of biochemical analysis, can solve the problems of inability to achieve high-throughput large-scale detection, cumbersome sample processing steps, and long experimental cycle time, achieving simple preparation methods, short separation time, reduced degradation and effect of loss

Active Publication Date: 2019-11-26
INST OF BOTANY CHINESE ACAD OF SCI
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Problems solved by technology

[0003] Only one of the above experimental methods has been verified on plant tissues, but the sample processing steps are cumbersome, which may easily cause the loss of target objects, and the experimental cycle is time-consuming, making it impossible to achieve high-throughput large-scale detection

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  • A method for rapid extraction and detection of geranylgeranyl pyrophosphate in plant tissues
  • A method for rapid extraction and detection of geranylgeranyl pyrophosphate in plant tissues
  • A method for rapid extraction and detection of geranylgeranyl pyrophosphate in plant tissues

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Embodiment Construction

[0031] The present invention will be described below through specific examples, but the present invention is not limited thereto.

[0032] The experimental methods used in the following examples are conventional methods unless otherwise specified; the reagents and materials used in the following examples can be obtained from commercial sources unless otherwise specified.

[0033] The present invention will be described in detail below in conjunction with the accompanying drawings. However, it should be understood that the accompanying drawings are provided only for better understanding of the present invention, and they should not be construed as limiting the present invention.

[0034] Such as figure 1 As shown, the present invention takes the plant tissue—the hairy root of Salvia miltiorrhiza as an example (the hairy root of Salvia miltiorrhiza is cultivated with reference to the literature [Li Bin, et al. Scientific Reports, 2017, 7:43320-43328.] Materials and Methods part...

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Abstract

The invention provides a method for rapidly extracting and detecting GGPP (geranylgeranyl pyrophosphate) in plant tissue. The method comprises the following steps of 1, extracting the plant tissue with a ternary mixed solvent system to obtain an extraction mixture; 2, centrifuging the extraction mixture and taking a supernatant liquid; 3, conducting chromatographic separation on the supernatant liquid and mass spectrometry on a compound obtained after separation. The method has the following advantages that the adopted preparation method of the ternary mixed rapid extraction solvent system issimple, and the system is easy to use and low in purchasing cost; the time of the ternary mixed rapid extraction solvent system for extracting the target compound GGPP is as short as one minute, and the degradation and loss of samples are reduced to the maximum extent. The adopted compound separation and detection system is short in separation time and low in detection sensitivity, the separationchromatogram and high resolution mass of the compound can be given within ten minutes, the physicochemical properties of the compound itself are retained to the maximum extent, and the degradation andloss of the samples are reduced.

Description

technical field [0001] The invention belongs to the technical field of biochemical analysis, in particular to a method for rapidly extracting and detecting geranylgeranyl pyrophosphate (GGPP) in plant tissues. Background technique [0002] GGPP is a class of isoprenoid intermediates with important biological activities produced in the mevalonate pathway, which plays a pivotal role in cell growth, differentiation, signal transduction, anti-inflammation, immune regulation, and anti-oxidation. In plants, GGPP is the precursor of carotenoids, gibberellins, vitamins, and chlorophyll, and is also a substrate for the construction of long-chain isoprenoids. As an intermediate product of biological samples, GGPP mostly exists in the form of ions, and its stability is poor at low concentrations. Therefore, it is urgent to establish simple and fast sample extraction and high-sensitivity detection and analysis methods. The prior art is mainly divided into the following categories: 1. A...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/89G01N30/72
CPCG01N30/02G01N30/72G01N30/89
Inventor 韩彬
Owner INST OF BOTANY CHINESE ACAD OF SCI
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