Method for in vitro mass amplification of human mature high-activity dendritic cells and application thereof

Abstract

The invention provides a method for preparing mature high-activity dendritic cells. The method comprises the following steps of (1) collecting single karyocytes from peripheral blood or umbilical cordblood; (2) inducing the single karyocytes to be differentiated into dendritic cells; (3) enabling the dendritic cells to be in contact with the non-human primate animal T lymphotropic virus protein from non-pathogenic; (4) screening and identifying the obtained mature high-activity dendritic cells. The invention provides the mature high-activity dendritic cell prepared by the method, and a use method of the cell. The dendritic cell prepared by the method has strong dendritic cell reproductive capability and high activity; the gene modification transmission is convenient; the cell is superiorto the dendritic cell used in the prior art. The cell and the use method have the wide application prospects in aspects of tumor or virus immunotherapy.

Description

technical field [0001] The invention relates to a method for preparing dendritic cells, especially a method for preparing mature highly active dendritic cells in vitro. The present invention also relates to mature highly active dendritic cells prepared by the method and related applications thereof. Background technique [0002] The immune system is the most important system for the human body to recognize and remove foreign pathogenic microorganisms (viruses) and mutated cells (tumors) in the body. The functional units that play a role in the immune system are various types of immune cells, including macrophages, B lymphocytes, T lymphocytes, natural killer cells (NK) and dendritic cells. Among them, B lymphocytes resist the invasion of foreign antigens by producing antibodies and mainly participate in humoral immunity; T lymphocytes and NK cells mainly mediate cellular immunity. As antigen-presenting cells, dendritic cells play a key role in cellular immunity mediated by...

AI Technical Summary

Problems solved by technology

This method is a commonly used clinical method at present, and has the following defects: First, the growth potential of MoDCs formed in vitro is limited and it is difficult to culture for a long time. Therefore, multiple rounds of vaccination need to be repeatedly prepared. Second, the mature and activated MoDCs in vitro are not CD141+DCs in the true sense, and their functions cannot be compared with primary DCs

But so far, there is no report that Tax protein of non-human primate T lymphocyte virus can induce dendritic cells to mature and maintain high activity in vitro