Daphnia magna counting device and using method thereof

Abstract

The invention relates to a daphnia magna counting device and a using method thereof. The device comprises an inner tube and an amplifying bottle, wherein the inner tube is withdrawable and is providedwith a rubber plug; the bottom of the inner tube is flat; and an anti-collision pad is arranged on the outer wall of the inner tube. A wear-resistant rubber ring is arranged on the opening of the amplifying bottle, the bottle body of the amplifying bottle is provided with a water adding scale mark, and an amplifying bottle anti-collision pad is arranged on the outer wall of a bottle bottom. Whenthe daphnia magna counting device is used, clear water is injected into the amplifying bottle to the water adding scale mark, a solution to be counted is poured into the inner tube, and the inner tubeis rapidly placed into the amplifying bottle. At the moment, daphnia magna in the solution of the inner tube gradually settles to the bottom of the inner tube, and an operator takes up the amplifyingbottle and observes the daphnia magna with light, and looks up from the bottom of the inner tube to count the number of the daphnia magna. Because the amplifying bottle which contains the clear watercan amplify the daphnia magna in the inner tube by 3-5 times, when the daphnia magna just enters the inner tube, the daphnia magna is scattered and swings from top to bottom, and the daphnia magna can be conveniently and accurately counted by the simple tool.

Description

technical field [0001] The invention relates to a zooplankton counting device, especially a fast counting device for daphna magna and its application method. Background technique [0002] Daphnia large ( Daphnia magna ) belongs to the phylum Arthropoda, Crustacea, and Cladocera, and is a common small planktonic crustacean, which can transfer pollutants through the food chain and play a vital role in the ecological environment. Due to its short life cycle, fast growth, high reproductive capacity, easy cultivation, and sensitivity to water environmental stress, Daphnia magna has become a model organism widely used in domestic and foreign laboratories for aquatic ecological testing. The results of acute activity inhibition and reproduction tests of Daphnia magna are important indicators for the classification of hazardous chemicals and the hazard assessment of new chemical substances in developed countries or regions such as the European Union, the United States, Japan, and my...

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