The invention relates to an immunohistochemical combined elastic fiber multi-staining kit, a staining method and application. The kit provided by the invention comprises the following components: an antigen repair solution, a Victoria blue dye solution, a peroxidase blocking agent, a primary antibody reagent, a secondary antibody reagent A, a secondary antibody reagent B, a DAB chromogenic substrate, a DAB chromogenic buffer solution, an HRP-Blue substrate, an HRP-Blue substrate buffer solution and a hematoxylin counterstaining solution. The primary antibody reagent is a mixed antibody mainly composed of an antibody A and an antibody B, the antibody A is a mouse monoclonal antibody, and the antibody B is a rabbit monoclonal antibody; the second antibody reagent A is an HRP (horse radish peroxidase) labeled goat anti-mouse polymer; the second antibody reagent B is an HRP (horse radish peroxidase) labeled goat anti-rabbit polymer. The invention also provides a specific dyeing method. According to the kit and the dyeing method, immunohistochemical double dyeing and elastic fiber dyeing are organically combined for the first time, the dyeing procedure is simplified, the detection efficiency is improved, and the demand for tissue samples is remarkably reduced.