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Genetic diversity evaluation method for sheep source group

A technology for genetic diversity and evaluation methods, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA preparation, etc., can solve the problems of low coverage and high cost of evaluation methods, achieve cost savings and improve comprehensiveness Effect

Inactive Publication Date: 2018-10-02
SICHUAN ACAD OF GRASSLAND SCI
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Problems solved by technology

[0006] The technical problem to be solved by the present invention is: in order to solve the problem of low coverage or high cost of existing evaluation methods, the present invention provides a method for evaluating genetic diversity of sheep resource populations. 26 pairs of autosomes and 30 microsatellite markers on the X sex chromosomes were used for allelic typing by capillary electrophoresis sequencing technology, comprehensively and systematically analyzed the genetic diversity of sheep resource populations, and then evaluated the genetic diversity of the populations, Provide molecular-level theoretical basis for the development and utilization of resource groups, improve the comprehensiveness of evaluation, and save costs

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  • Genetic diversity evaluation method for sheep source group
  • Genetic diversity evaluation method for sheep source group
  • Genetic diversity evaluation method for sheep source group

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Embodiment Construction

[0023] 1) Blood sample collection

[0024] Using the Butuo black sheep in Butuo County, Liangshan Yi Autonomous Prefecture as the test material, 3-4 mL of blood was collected from the jugular vein, injected into a disposable vacuum blood collection tube added with anticoagulant heparin sodium, and then the blood collection tube was mixed upside down at 180° for 5 -6 times, transported in a low-temperature storage box, and brought back to the laboratory for long-term storage at -20°.

[0025] 2) DNA extraction

[0026] DNA was extracted using the TIANNamp Genomic DNAkit kit produced by Tiangen Biochemical Technology (Beijing) Co., Ltd., and the steps were as follows: 1) Take 200 μL of blood sample, add it to a 1.5 mL EP tube, add 20 μL of proteinase K solution, mix well, and then add 200 μL of buffer solution GB, fully inverted and mixed, placed in a 70°C water bath for 10 minutes; 2) Take out the EP tube, add 200 μL of absolute ethanol, shake and mix well for 15 seconds, tran...

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Abstract

The invention relates to the field of genetic diversity evaluation methods, in particular to a genetic diversity evaluation method for a sheep source group. The method comprises the following steps: a, collecting a blood sample of sheep; b, extracting a genome DNA of the blood sample; c, screening 30 pairs of microsatellite markers from a microsatellite marker database, distributing into 26 pairsof autosomes and the X sex chromosome of the sheep, verifying the sequence of the primer through an NCBI database, using 5-ROX, 5'6-FAM(FITC), 5-TAMRA and 5-HEX to label fluorescence on the microsatellite marker; d, carrying out PCR amplification and allelic discrimination of the microsatellite marker; e, carrying out statistical analysis, and evaluating the genetic diversity of the resource group. The method comprehensively systemically analyzes and evaluates the genetic diversity of the sheep source group, provides theoretical basis for the development and utilization of the sheep resource group on the molecular level, improves the comprehensiveness of the evaluation, and saves the cost.

Description

technical field [0001] The invention relates to the field of genetic diversity evaluation methods, in particular to the genetic diversity evaluation method of sheep resource groups. Background technique [0002] Biodiversity refers to the diversity of all organisms (animals, plants, microorganisms, etc.) on the earth, the genes they contain, and the ecosystem formed by the interaction between these organisms and the environment, including species Diversity, genetic diversity and ecosystem diversity. Genetic diversity can be expressed at multiple levels, such as molecules, cells, individuals, etc. During the long-term evolution of organisms, the change (or mutation) of genetic material is the root cause of genetic diversity, that is, the number and structure of chromosomes Variations and changes in nucleotides within genetic loci exhibit various phenotypes. Genetic diversity is an important part of biodiversity. The differences between individuals within a species are the r...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12Q1/6869
CPCC12Q1/6888C12N15/1006C12Q1/686C12Q1/6806C12Q2600/156C12Q2600/124C12Q2525/151
Inventor 周明亮杨平贵庞倩陈明华蒋世海
Owner SICHUAN ACAD OF GRASSLAND SCI
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