Whole genome sequence of foot and mouth disease virus artificial attenuated virus strain B, and primer and application thereof

A foot-and-mouth disease virus and whole genome technology, applied in the direction of application, virus, virus peptide, etc., can solve the biological problems such as inability to rescue the virus, incomplete identification of serotype foot-and-mouth disease virus sequence, inability to understand the virus growth curve, virus titer pathogenicity, etc. Characteristics and other issues, to achieve the effect of broad research and industrialization prospects

Active Publication Date: 2018-10-12
VETERINARY INST XINJINAG ACADEMY OF ANIMAL SCI CLINIC MEDICAL SCI RES CENT XINJIANG ACADEMY OF ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The different topological sequences of each serotype of FMD virus are incomplete, making it difficult to study the molecular pathogenic mechanism of different topological FMD viruses
Because there is no report on the complete genome sequence of the artificially attenuated strain of FMD virus O / Akesu / 58 CE39B, it is impossible to understand the biological characteristics of the virus such as growth curve, virus titer, and pathogenicity, and it is also impossible to use reverse genetics. OS Gets Rescue Virus

Method used

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  • Whole genome sequence of foot and mouth disease virus artificial attenuated virus strain B, and primer and application thereof
  • Whole genome sequence of foot and mouth disease virus artificial attenuated virus strain B, and primer and application thereof
  • Whole genome sequence of foot and mouth disease virus artificial attenuated virus strain B, and primer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: The whole genome sequence of artificially attenuated foot-and-mouth disease virus strain O / Akesu / 58 CE39B, as shown in SEQ ID No.1.

Embodiment 2

[0027] Embodiment 2: the assay method of foot-and-mouth disease virus artificial attenuation strain O / Akesu / 58 CE39B complete genome sequence, carry out as follows: the first step, with foot-and-mouth disease virus artificial attenuation strain O / Akesu / 58 CE39B cDNA as template, Use the primers shown in SEQ ID No.2 to SEQ ID No.15 to amplify 7 fragments of the full genome of foot-and-mouth disease virus artificially attenuated strain O / Akesu / 58 CE39B; the second step, after the amplification, the target fragments are recovered and TA Cloning identification, connecting into the sequencing vector pMD19-T for sequencing; the third step, after sequencing, sequentially splicing, editing and correcting the overlapping parts of the DNA sequences of the 7 fragments to obtain the artificially attenuated strain of foot-and-mouth disease virus O / Akesu / 58 CE39B full genome sequence.

Embodiment 3

[0028] Example 3: Primers for amplifying the whole genome sequence of artificially attenuated foot-and-mouth disease virus strain O / Akesu / 58 CE39B, the sequences of which are shown in SEQ ID No.2 to SEQ ID No.15.

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Abstract

The invention relates to the technical field of molecular biology, in particular to a whole genome sequence of a foot and mouth disease virus artificial attenuated virus strain O / Akesu / 58 CE39B, and aprimer and application thereof. The whole genome sequence of the foot and mouth disease virus artificial attenuated virus strain O / Akesu / 58 CE39B is shown in SEQ ID No.1. The whole genome sequence ofthe foot and mouth disease virus artificial attenuated virus strain O / Akesu / 58 CE39B is disclosed for the first time, facilitates virus reverse genetic research, lays the foundation for further research of the proliferative mechanism, the virus titer, the virulence mechanism and FMDV marker vaccines of FMDV in cells, and facilitates researching of virus evolution and virulence, molecular epidemiology and the like. The foot and mouth disease virus artificial attenuated virus strain O / Akesu / 58 CE39B has good safety and effectiveness in virus rescue, and is expected to serve as a genetic engineering vaccine of the next generation to be used for foot-and-mouth disease prevention and control, and the whole genome sequence has the wide research and industrialization prospects.

Description

technical field [0001] The invention relates to the technical field of molecular biology, and relates to a full genome sequence of the artificially attenuated strain O / Akesu / 58CE39B of foot-and-mouth disease virus. Application; also includes a method for determining the whole genome sequence of the artificially attenuated strain O / Akesu / 58 CE39B of foot-and-mouth disease virus; also includes a method for amplifying the whole genome sequence of the artificially attenuated strain O / Akesu / 58 CE39B of the foot-and-mouth disease virus The primers are used as preparations or kits for preparing and amplifying the artificial attenuated strain O / Akesu / 58 CE39B genome sequence of foot-and-mouth disease virus; it also includes an artificially attenuated strain O / Akesu / 58 CE39B of foot-and-mouth disease virus , the application of the artificially attenuated strain of foot-and-mouth disease virus O / Akesu / 58 CE39B in rescuing O-type foot-and-mouth disease virus, preparing a vaccine for prev...

Claims

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Application Information

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IPC IPC(8): C12N15/42C12Q1/6869C12N15/11C12N7/00C12N7/04A61K39/135A61P31/14
CPCA61K39/12A61P31/14C12N7/00C12Q1/6869C07K14/005C12N2770/32121C12N2770/32122C12N2770/32134C12N2770/32162C12Q2531/113
Inventor 苗书魁马文戈汪萍魏玉荣米晓云魏婕夏俊陆桂丽沙依兰·卡依扎王延薛英黄炯
Owner VETERINARY INST XINJINAG ACADEMY OF ANIMAL SCI CLINIC MEDICAL SCI RES CENT XINJIANG ACADEMY OF ANIMAL HUSBANDRY SCI
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