Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method of ligand-gated diagnosis and treatment system and application of ligand-gated diagnosis and treatment system in real-time tumor response

A gating and ligand technology, applied in the fields of biotechnology and pharmacy, can solve problems such as increasing the complexity of synthesis, reducing clinical application, increasing the difficulty of drug loading, etc., and achieves reliable and reliable diagnosis, good therapeutic effect, and timely and effective treatment. Effect

Active Publication Date: 2018-11-02
FUDAN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the optical transparency of commonly used mesoporous silica nanoparticles, the bound fluorescent probes can only be quenched by further surface or pore modification, which not only increases the synthesis complexity and reduces the potential of clinical application, It also increases the difficulty of drug loading (combination of diagnosis and treatment)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of ligand-gated diagnosis and treatment system and application of ligand-gated diagnosis and treatment system in real-time tumor response
  • Construction method of ligand-gated diagnosis and treatment system and application of ligand-gated diagnosis and treatment system in real-time tumor response
  • Construction method of ligand-gated diagnosis and treatment system and application of ligand-gated diagnosis and treatment system in real-time tumor response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Mesoporous carbon nanospheres were treated with a mixture of sulfuric acid and nitric acid (volume ratio 2:1), ultrasonicated for 2 hours, stirred at 50°C for 2 hours, centrifuged, washed with water, and dried to obtain oxidized mesoporous carbon nanospheres OMCN. Mix OMCN (400μg / mL) with Dox (20μg / mL), stir at 300rpm for 6h, then add P 0 (2μmol / L) stirred for 1h to obtain Dox-OMCN-P 0 Ligand-gated diagnosis and treatment system, stored at 4°C for future use.

Embodiment 2

[0095] Mesoporous carbon nanospheres were treated with a mixture of sulfuric acid and nitric acid (volume ratio 3:1), ultrasonicated for 2 hours, stirred at 50°C for 2 hours, centrifuged, washed with water, and dried to obtain oxidized mesoporous carbon nanospheres OMCN. Mix OMCN (400μg / mL) with Dox (20μg / mL), stir at 300rpm for 6h, then add P 0 (2μmol / L) stirred for 1h to obtain Dox-OMCN-P 0 Ligand-gated diagnosis and treatment system, stored at 4°C for future use.

Embodiment 3

[0097] Mesoporous carbon nanospheres were treated with a mixture of sulfuric acid and nitric acid (volume ratio 4:1), ultrasonicated for 2 hours, stirred at 50°C for 2 hours, centrifuged, washed with water, and dried to obtain oxidized mesoporous carbon nanospheres OMCN. Mix OMCN (400μg / mL) with Dox (20μg / mL), stir at 300rpm for 6h, then add P 0 (2μmol / L) stirred for 1h to obtain Dox-OMCN-P 0 Ligand-gated diagnosis and treatment system, stored at 4°C for future use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biotechnology and pharmaceuticals, and relates to a preparation method of an OMCN-based efficient and sensitive ligand-gated diagnosis and treatment system. According to the invention, multiple diagnosis and treatment of tumors in vivo and in vitro can be realized by specifically binding MUC1 highly expressed on the surface of malignant tumors; the OMCN-based ligand-gated diagnosis and treatment system provided by the invention has low detection limit, can be applied to the detection and treatment of different levels of MUC1 molecules, tumor cells and living tumor tissues, ensures an authentic and reliable diagnosis, and is efficient in targeted treatment. The invention provides an economical, practical and simple preparation technology of the OMCN-based ligand-gated diagnosis and treatment system, so as to realize the multiple diagnosis and treatment of the tumors in vitro and in vivo.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and pharmacy, relates to a drug-carrying system, in particular to a construction method of a ligand-gated diagnosis and treatment system and its application in real-time tumor response. Background technique [0002] Data show that in recent years, although people have invested a lot of scientific research in the treatment of cancer, a major disease, the mortality rate of cancer patients has not been significantly improved. At present, the treatment of tumors in clinical practice is still confined to the mode of separation of diagnosis and treatment. The survey shows that defects such as false positive / negative diagnosis, late diagnosis, tumor metastasis, and off-target side effects still exist. In fact, targeted controllable / on-demand drug delivery based on real-time diagnosis is an ideal tumor treatment mode, which can realize early and efficient diagnosis of tumors, accurately deliver drug...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/533G01N33/543G01N21/64
CPCG01N21/6428G01N33/533G01N33/54346G01N33/57484G01N2021/6439G01N2021/6432
Inventor 黄容琴王义李诚意
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com