Low-temperature dimethyl phthalate high efficiency degrading bacterial strain, and fermentation method thereof

Abstract

The invention discloses a low-temperature dimethyl phthalate high efficiency degrading bacterial strain, and a fermentation method thereof. The low-temperature dimethyl phthalate high efficiency degrading bacterial strain is pseudomonas putida, is named as pseudomonas putida QDF12, is preserved at China Center for Type Culture Collection, Wuhan university, and the preservation number is CCTCC NO.M2018272. It is confirmed by experiments that at 15 DEG C low temperature environment, in DMP solution of mass concentration of 1000mg / L, the degradation rate on DMP at 48h is 96.895%, the degradationrate is as high as 20.1855mg / L.h. The fermentation conditions of the low-temperature dimethyl phthalate high efficiency degrading bacterial strain are optimized to increase QDF12 bacteria cell fermentation yield, after optimization, the fermentation biomass is increased obviously, and is increased by 374.36% at most, and at the same time, fermentation time is shortened to 12h, the fermentation temperature is as low as 11.86 DEG C. It is shown that the low temperature DMP degradation bacterial strain QDF12 possesses rapid, high efficiency, low energy consumption fermentation characteristics under optimized fermentation conditions, cost of large-scale production is reduced, and benefit effect is improved.

Description

technical field [0001] The invention relates to a strain of dimethyl phthalate (DMP) degrading bacteria and a fermentation method thereof, in particular to a strain of Pseudomonas putida that can efficiently degrade high-concentration DMP under low temperature conditions. The invention belongs to the field of biotechnology. Background technique [0002] Phthalate esters (PAEs) are a class of commonly used plasticizers, including more than 20 organic compounds, widely found in plastic products, food, daily chemical products, cosmetics, and medicine. PAEs act like estrogen, and humans and other organisms enter the body through ingestion and skin contact to cause serious diseases. For example, harming the secretion of normal human hormones leads to endocrine disorders and a series of diseases; the reproductive toxicity and genotoxicity caused by chromosomal aberrations cause birth defects, organ damage, infertility and tumors. Since the plasticizer incident in Taiwan in 2011,...

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