Comparative hybrid population sampling method for establishing transgenic drift environment safety detection

An environmentally safe and transgenic technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of not being able to truly and accurately reflect fitness effects, inconsistency in genetic backgrounds, and inconsistency in parental allele partial segregation. and other problems, to achieve good application prospects, reduce genetic background inconsistencies, and reduce errors.

Active Publication Date: 2018-11-13
FUDAN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, this traditional sampling method can lead to differences in the partial segregation of parental alleles and genetic background inconsistencies between the two comparison populations, that is, the population containing the transgene has more crop alleles, while the Populations without the transgene have more CWR alleles
In addition to the difference in the presence or absence of genetically modified genes, the two groups are also inconsistent in genetic background, which leads to the fact that the results of fitness evaluation cannot truly and accurately reflect the fitness effect brought about by genetically modified genes.

Method used

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  • Comparative hybrid population sampling method for establishing transgenic drift environment safety detection
  • Comparative hybrid population sampling method for establishing transgenic drift environment safety detection
  • Comparative hybrid population sampling method for establishing transgenic drift environment safety detection

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Experimental program
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Effect test

Embodiment 1

[0017] Insect-resistant transgenes constructed using traditional sampling methods CpTI (Positive population, P+) and no insect-resistant transgene CpTI (Negative population, P-) comparative experimental population, in the process of genetic analysis of the P+ population and P- population, it was found that the P+ and P- comparison populations showed the frequency of alleles from their parents at some SSR loci Different, with partial segregation phenomenon, further calculate the genetic differentiation index between P+ and P-comparison groups ( F ST ) values ​​indicate that there are significant differences in the genetic background between the comparison groups ( figure 1 ), such differences will seriously affect the resistance to insect transgenics CpTI Results of the analysis of the fitness effect.

[0018] Constructs containing insect-resistant transgenes using traditional sampling methods CpTI (Positive population, P+) and no insect-resistant transgene CpTI (Neg...

Embodiment 2

[0038] Using the sampling method of the present invention to construct insect-resistant transgenes CpTI (Positive population, P+) and no insect-resistant transgene CpTI (Negative population, P-) compared to the experimental population. During the genetic analysis of the P+ population and the P- population, it was found that at some SSR loci, the P+ and P-comparison populations showed partial segregation of the alleles from the parents, but the P+ population and the P-comparison population The genetic differentiation index between ( F ST ) values ​​are very small, indicating that the genetic background between the P+ population and the P- population is very close ( figure 2 ), the detected fitness difference can truly reflect the difference brought about by the transgene, which is beneficial to the CpTI Proper assessment of the environmental risk of insect-resistant transgene drift into crop wild relatives.

[0039] Using the sampling method of the present invention to...

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Abstract

The invention belongs to the technical field of environmental risk assessment of genetically modified organisms and particularly relates to a comparative hybrid population sampling method for establishing transgenic drift environment safety detection. The method includes utilizing a transgene from a crop and a neutral molecular marker from the same chromosome of the crop but close to the transgeneand without physical linkage for sampling, respectively constructing a hybrid progeny separating experiment population containing the transgene or not containing the transgene, making the genetic background of the two populations infinitely close, and then conducting conformity correlated character analysis and comparison on the two populations under the common garden environmental conditions soas to accurately predict and evaluate whether the transgene entering wild relatives of the crop through natural hybridization will lead to environmental risks or not. By means of the method, the difference in allelic partial segregation between the comparative populations (P+ and P-) due to sampling and the inconsistency of the genetic background are greatly reduced, so that a result of the safetyevaluation of transgenic organisms is closer to the real situation.

Description

technical field [0001] The invention belongs to the technical field of environmental risk assessment and analysis of genetically modified organisms, and in particular relates to a comparative hybrid population sampling method for establishing environmental safety detection of transgenic drift. Background technique [0002] The rapid development of genetically modified technology has continuously promoted the cultivation of genetically modified plants. The commercial planting of genetically modified crops has provided new ways to increase crop yields, reduce pesticide use, improve the agricultural ecological environment, and solve food security problems. However, transgenes may escape from crops to their wild relative populations through gene drift, which may pose certain environmental risks. Therefore, before the commercialization of transgenic crops, the potential environmental risks that may be caused by transgenic drift must be detected and evaluated to ensure the safe an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/24
CPCC12Q1/24
Inventor 蔡星星卢宝荣王哲汪智
Owner FUDAN UNIV
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