Epidermal stem cell RAB23 gene knockout through CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) system

A technology of epidermal stem cell and gene editing, applied in the field of RAB23 gene editing, can solve the problem of siRNA not stable inheritance, etc., and achieve the effect of high knockout efficiency, stable passage, and strong knockout effect
CN108893473AActive Publication Date: 2018-11-27浙江玉安康瑞生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
浙江玉安康瑞生物科技有限公司
Publication Date
2018-11-27

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Abstract

The invention provides epidermal stem cell RAB 23 gene editing through a CRISPR-Cas system and particularly relates to establishment of an RAB23 gene knockout epidermal stem cell system for subsequentresearch on cutaneous squamous cell carcimona. According to the establishment of an RAB23 gene knockout epidermal stem cell system, a specific gRNA (guide ribonucleic acid) is structured and obtainedto significantly improve epidermal stem cell intracellular CRISPR / Cas9 RAB23 gene editing. A provided epidermal stem cell RAB23 knockout plasmid is high in hereditary stability and targeting efficiency.
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Description

technical field

[0001] The present invention provides a CRISPR-Cas system for RAB23 gene editing on epidermal stem cells, and in particular relates to the establishment of a RAB23 knockout epidermal stem cell line. Background technique

[0002] Epidermal stem cells (Epidermal stem cells, EpiSCS) are stem cells with self-proliferation and multi-lineage differentiation potential. Its normal proliferation and differentiation are the basic requirements for maintaining the structural and functional integrity of the skin and its appendages (sweat glands, hair, sebaceous glands). Under physiological conditions, epidermal stem cells differentiate into a stem cell and a transit amplifying cell (TA cell) through asymmetric division, and the TA cell differentiates into post-mitotic cells (Post-mitotic cells) and terminal cells after multiple divisions. Differentiated cells (terminally-differentiated cells) to supplement the continuous renewal of epidermal cells. Studies have shown tha...

Claims

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