Ging2 gene knockout in epidermal stem cells using CRISPR-Cas system

Abstract

The invention provides a method using a CRISPR-Cas system to perform GING2 gene editing on an epidermal stem cell and particularly relates to a method for building an epidermal stem cell line with theGING2 gene being knocked out. Two specific gRNAs are built and acquired, and the GINS2 gene editing efficiency of CRISPR / Cas9 in the epidermal stem cell can be increased evidently. An epidermal stemcell GINS2 knockout plasmid is good in hereditary stability and high in targeting efficiency.

Description

technical field

[0001] The present invention provides a GING2 gene editing method for epidermal stem cells by using a CRISPR-cas system, and in particular relates to the establishment of a GING2 gene knockout epidermal stem cell line. Background technique

[0002] Epidermal stem cells (Epidermal stem cells, EpiSCS) are stem cells with self-proliferation and multi-lineage differentiation potential. Its normal proliferation and differentiation are the basic requirements for maintaining the structural and functional integrity of the skin and its appendages (sweat glands, hair, sebaceous glands). Under physiological conditions, epidermal stem cells differentiate into a stem cell and a transit amplifying cell (TA cell) through asymmetric division, and the TA cell differentiates into post-mitotic cells (Post-mitotic cells) and terminal cells after multiple divisions. Differentiated cells (terminally-differentiated cells) to supplement the continuous renewal of epidermal cells. St...

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