Anti-browning culture medium and anti-browning tissue culture method of peony in south of Yangtze river

Abstract

The invention provides a culture medium containing an anti-browning agent. Browning of peony in south of Yangtze river in growth in culture medium can be controlled effectively through selection of different concentrations of the anti-browning culture medium at different stages, the growth of peony in south of Yangtze river is not influenced in prevention of browning; and in addition, a transpiration inhibitor is sprayed onto tissue culture plants of peony in south of Yangtze river, so that water evaporation is inhibited, and growth is promoted.

Description

technical field

[0001] The invention relates to a culture medium and a tissue culture method of Jiangnan peony, in particular to an anti-browning culture medium and an anti-browning tissue culture method of Jiangnan peony. Background technique

[0002] Jiangnan peonies have the same ecological habits as other peonies, such as light-loving and fertile soil. The climate without severe summer heat and winter is more suitable for the growth of peony, but compared with other peonies, Jiangnan peony is more resistant to "high temperature and high humidity" climate, high water transpiration during growth. In recent years, due to factors such as global warming and no major breakthroughs in breeding methods, the overall variety of Jiangnan peonies has been decreasing year by year. Up to now, there are less than 30 varieties, and the extinction of varieties is very serious. In the tissue culture method of Jiangnan peony, strong water transpiration and browning are unavoidable, and no...

Examples

Embodiment Construction

[0010] All samples were selected according to the interval of 0.3mg / L, and the silver nitrate content of the selected samples were 0.5mg / L, 0.8mg / L, 1.1mg / L, 1.4mg / L, 1.7mg / L, 2.0mg / L, 2.3mg / L, 2.6mg / L, 2.9mg / L, 3.2mg / L, 3.5mg / L, 3.8mg / L, 4.1mg / L, 4.4mg / L, 4.7mg / L, 5.0mg / L, 5.3mg / L, 5.6mg / L, 5.9mg / L, 6.2mg / L, 6.5mg / L, 6.8mg / L, 7.1mg / L, 7.4mg / L, 7.7mg / L, 8.0mg / L, 8.3mg / L, 8.6mg / L, 8.9mg / L, 9.2mg / L, 9.5mg / L, 9.8mg / L, 10.1mg / L, 10.4mg / L, 10.7mg / L, 11.0mg / L, 11.3mg / L, 11.6mg / L, 11.9mg / L, 12.2mg / L, 12.5mg / L, 12.8mg / L, 13.1mg / L, 13.4mg / L, 13.7mg / L, 14.0mg / L culture medium , and then select the medium without adding any anti-browning agent as a reference.

[0011] Then, the cultivation time of the tissue cultured seedlings in the anti-browning medium is divided into two stages, the first stage is the 1st-12th day, and the second stage starts from the 13th day, allowing Jiangnan peonies to grow up to second stage.

[0012] When the silver nitrate content is 1.7mg / L-2.6mg / L, th...

technical field

[0001] The invention relates to a culture medium and a tissue culture method of Jiangnan peony, in particular to an anti-browning culture medium and an anti-browning tissue culture method of Jiangnan peony. Background technique

[0002] Jiangnan peonies have the same ecological habits as other peonies, such as light-loving and fertile soil. The climate without severe summer heat and winter is more suitable for the growth of peony, but compared with other peonies, Jiangnan peony is more resistant to "high temperature and high humidity" climate, high water transpiration during growth. In recent years, due to factors such as global warming and no major breakthroughs in breeding methods, the overall variety of Jiangnan peonies has been decreasing year by year. Up to now, there are less than 30 varieties, and the extinction of varieties is very serious. In the tissue culture method of Jiangnan peony, strong water transpiration and browning are unavoidable, and no...

Examples

Embodiment Construction

[0010] All samples were selected according to the interval of 0.3mg / L, and the silver nitrate content of the selected samples were 0.5mg / L, 0.8mg / L, 1.1mg / L, 1.4mg / L, 1.7mg / L, 2.0mg / L, 2.3mg / L, 2.6mg / L, 2.9mg / L, 3.2mg / L, 3.5mg / L, 3.8mg / L, 4.1mg / L, 4.4mg / L, 4.7mg / L, 5.0mg / L, 5.3mg / L, 5.6mg / L, 5.9mg / L, 6.2mg / L, 6.5mg / L, 6.8mg / L, 7.1mg / L, 7.4mg / L, 7.7mg / L, 8.0mg / L, 8.3mg / L, 8.6mg / L, 8.9mg / L, 9.2mg / L, 9.5mg / L, 9.8mg / L, 10.1mg / L, 10.4mg / L, 10.7mg / L, 11.0mg / L, 11.3mg / L, 11.6mg / L, 11.9mg / L, 12.2mg / L, 12.5mg / L, 12.8mg / L, 13.1mg / L, 13.4mg / L, 13.7mg / L, 14.0mg / L culture medium , and then select the medium without adding any anti-browning agent as a reference.

[0011] Then, the cultivation time of the tissue cultured seedlings in the anti-browning medium is divided into two stages, the first stage is the 1st-12th day, and the second stage starts from the 13th day, allowing Jiangnan peonies to grow up to second stage.

[0012] When the silver nitrate content is 1.7mg / L-2.6mg / L, th...