31results about How to "Growth is not inhibited" patented technology

The invention relates to the field of plant remediation technology for soil contaminated by PAHs, specifically to a remediation method for soil contaminated by PAHs by using the gramineous plant Festuca arundinacea. The gramineous plant Festuca arundinacea is planted in soil contaminated by PAHs; in the process of germination, growth and maturation of the plant, PAHs in the soil are degraded, removed or substantially reduced through combined action of the growth process and root exudates of the plant. According to the method, the ornamental plant Festuca arundinacea is planted in the soil contaminated by PAHs for remediation of the soil contaminated by PAHs; compared with a traditional contaminated soil treatment method, the method provided by the invention has the advantages of small investment, small work amount, low technical requirements, etc. Moreover, as Festuca arundinacea is an ornamental and economic plant, planting of Festuca arundinacea enables PAHs contamination in environmental soil to be effectively degraded and contaminated plants not to enter into a food chain, thereby utmostly reducing ecological risks caused by treatment of the contaminated soil.

The invention belongs to the field of plant tissue culture, and particularly relates to a method for promoting rooting of a butterfly orchid tissue cultured seedling by utilizing an LED light source. The method specifically comprises the following steps: (1) performing induced culture by taking a butterfly orchid flower as an explant to obtain a cluster bud; (2) multiplying and culturing the cluster bud obtained in the step (1), dividing the multiplied and cultured cluster buds into single buds and inoculating the single buds into a rooting culture medium; (3) culturing and rooting the cluster buds which are inoculated into the rooting culture medium in mixed light consisting of three single-color lights including red light, blue light and far-red light. According to the method, the treatment of the mixed light added with the far-red light promotes the growth of the roots of butterfly orchid seedlings, so that accumulation of dry matters is increased, and the growth of the underground parts is not inhibited; meanwhile, the method has the advantages of lowering the production cost and saving energy.

The invention provides a method of repairing shale gas fracturing waste liquid. The method includes steps of transplanting seedlings of Oenanthe javanica into the shale gas fracturing waste liquid; adding multiple microorganisms and culturing the Oenanthe javanica until blossom; and removing the Oenanthe javanica. The method has strong operability and low cost, wherein the plant and the microorganisms are easy to obtain. The method also can clean and beautify environment.

The invention provides an in vitro detection kit for anaerobic microorganisms, which relates to the technical field of biochemical detection. The kit includes a culture solution, an adsorption resin, a culture bottle body, a bottle cap and a receptor; the culture solution includes tryptone, Gelatin peptone, brain heart infusion, L-arginine, L-cysteine ​​hydrochloride, active synergist, sodium citrate, monopotassium phosphate and vitamin C. The kit can effectively neutralize antibiotics and provide more favorable growth conditions for microorganisms, thereby increasing the positive detection rate of anaerobic microorganisms and shortening the detection time.

The invention provides a method for controlling the large-scale cultivation of chlorella polluting bacteria, by adding plant extracts to the chlorella culture system to control and / or reduce the number of other polluting bacteria, thereby promoting the growth of chlorella grow. The plant extract comprises knotweed extract and agarwood extract, and the weight / number ratio of the knotweed extract to the agarwood extract is 1:8-20. The present invention adopts Polygonum cuspidatum extract and Agarwood extract in the culture system of Chlorella, which not only removes miscellaneous bacteria, but also increases the anti-pollution performance of Chlorella, and the Polygonum cuspidatum extract can promote the growth and antibacterial properties of Chlorella. Agarwood extract has no inhibitory effect on the growth of Chlorella.

The invention provides a method for producing pig-source adiponectin. The method includes the steps that operon capable of carrying out secretory expression on sumo protease and operon capable of carrying out secretory expression on saccharomyces cerevisiae small-molecule ubiquitin protein and pig-source adiponectin fusion protein are artificially synthesized, then carriers pZLS-128 through which pig-source adiponectin maternal genes can be expressed in bacillus subtilis are obtained through cloning, and enter competent cells of bacillus-subtilis WB700 strains in an electrotransformation mode, recombined bacillus-subtilis BZLS128 strains are obtained, and pig-source adiponectin protein can be obtained after the recombined bacillus-subtilis BZLS128 strains are subjected to induction fermentation in a flask. The method has the advantages of being simple in expression system, capable of being used for large-scale production, low in production cost and the like; necessary technical supports are provided for commercial application of the pig-source adiponectin.

The invention provides an application of a pyridyl pyrimidinol compound in inducing plant resistance. The compound can significantly and rapidly improve the ability of plants to resist pathogenic bacteria infection, the active oxygen signal appears earlier, reduces the cell death caused by plant infection by pathogenic bacteria, and the growth of pathogenic bacteria in plants is significantly inhibited to protect plants from pathogenic bacteria. It will inhibit the growth of plant roots and seedlings, but has a slight growth-stimulating effect on plants, can promote the growth and development of lateral roots of plants, and will not cause secondary pollution of pesticides to the environment, so it has broad application value.

The invention relates to a growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and a using method of the growth promoting agent. The growth prompting agent is obtained as follows: adding 1 part weight of one or a mixture of sorbic acid, potassium sorbate or sodium sorbate to 1-4 parts by weight of alcohol for stirring, mixing and dissolving. The using method of the growth promoting agent is as follows: adding the growth promoting agent to a prepared photosynthetic bacteria liquid medium for stirring until the growth promoting agent is sufficiently dissolved; adding active photosynthetic bacteria strains for filling to a sealed or semi-open light transmission container for culturing at 25-32 DEG C by radiating under 1500lux-1600lux, wherein sorbate radial concentration of the growth promoting agent, which is added to the photosynthetic bacteria liquid medium, is controlled within a range of 0.005 mmol / L-1.0 mmol / L. The growth promoting agent for promoting photosynthetic bacteria strain to quickly breed is simple to use, less in usage, low in cost and capable of promoting photosynthetic bacteria strain to quickly breed. Moreover, the using method of the growth promoting agent is safe, ecological and environment-friendly and extensive in market prospect.

The invention discloses a preparation method of N-doped carbon quantum dots, and a product and application thereof. The method comprises the following steps: dissolving chitosan in dionized water, placing in a hydrothermal reaction kettle at certain temperature to react, after a synthetic product is naturally cooled, separating, purifying, freeze drying and obtaining powder of the N-doped carbon quantum dots. Compared with other preparation method of the carbon quantum dots, the preparation method of the invention is simple in operation, only needs on raw material, needs no other surface passivating agent and is high in reaction speed, the prepared carbon quantum dots have high fluorescence and good biological compatibility, and a yield of the carbon dots reaches 45 percent. The carbon quantum dots not only can promote the growth of plants, but also can mark the plants. The application prospect in the fields of biology and agriculture is wide in the future.

The invention relates to a rapid sterilization method in the conventional culture process of kelp gametophyte clones. Pour the gametocyte clones with serious bacterial contamination together with the culture solution into a sterilized container, then move the sterilized circulation pump into the container, and inflate and disperse the bacteria. Attach the clonal group of bacteria, then let it stand and change the water to separate and remove the bacteria. The biggest feature of the method of the invention is that it is easy to operate, fast and efficient; through the circulation pump, it kills two birds with one stone, not only removing bacteria, but also breaking up clone groups, which is beneficial to the faster growth of gametocytes.