Fast degerming method in process of conventional culture of kelp gametophyte cloning

A gametophyte and rapid technology, applied in plant cells, chemistry, etc., can solve the problem of time-consuming and labor-intensive washing methods, and achieve the effect of easy operation

Active Publication Date: 2020-11-20
SHANDONG ORIENTAL OCEAN SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for rapid sterilization of kelp gametophyte clones in the conventional culture process, to overcome the time-consuming and labor-intensive defects of the existing high-pressure water gun flushing method, and to realize rapid and efficient sterilization of kelp gametophyte clones

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  • Fast degerming method in process of conventional culture of kelp gametophyte cloning

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Embodiment Construction

[0016] Below in conjunction with embodiment further illustrate the present invention.

[0017] 1. Selected materials: gametophyte clones with serious bacterial contamination. The method for distinguishing the serious bacteria attached: when the surface of the gametophyte clone is gray and unclean, and the color of the clone is not bright, further observation through the microscope shows that bacteria are attached to the surface of each branch of the gametophyte clone cluster, or the clone group is wrapped by bacteria. Such as figure 1 .

[0018] 2. Disinfection of circulation pumps: put six circulation pumps into a 5000mL conical flask filled with 75% alcohol at the same time, immerse the connection part of the circulation pumps in the alcohol, turn on the power supply, fully disinfect for 1min, and put the circulation pumps and connections Move the thread into conical flasks filled with fresh water (boiled and cooled) and seawater (boiled and cooled) in turn, inflate for 1 ...

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Abstract

The invention relates to a fast degerming method in a process of conventional culture of kelp gametophyte cloning. The fast degerming method in the process of conventional culture of kelp gametophytecloning comprises the steps of pouring gametophyte clone groups with serious bacterial attachment into a disinfected container along with culture liquid, then transferring disinfected circulating pumps into the container, conducting inflation to break up the clone groups where bacteria are attached, then allowing standing, and changing water to separate out the bacteria. The most important characteristics of the method are that the method is simple and convenient to operate, fast and efficient; and the circulating pumps have double benefits, wherein not only are the bacteria removed, but alsothe clone groups are broken up, so that faster growth of gametophytes is facilitated.

Description

technical field [0001] The invention relates to a method for eliminating bacteria of kelp gametophyte clone. Background technique [0002] Kelp is one of the important economically cultivated seaweeds in my country. With the establishment of kelp gametophyte cloning technology and the continuous improvement of the gametophyte cloning seedling breeding technology system, gametophyte cloning seedling breeding has become an inevitable trend of kelp seedling breeding. Cloning seedlings can solve many disadvantages of spore seedlings, and has the following technical advantages: omit the cultivation and selection of kelp grown at sea, and reduce labor costs; seedlings can be harvested at any time without being restricted by sea areas; seedling harvesting time can be delayed, Shorten the seedling time and reduce the cost of refrigeration; because the genetic basis of gametophyte cloning is pure, the excellent traits of the variety can be maintained for a long time; the heterosis o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A61L2/18A61L101/34
CPCC12N5/04A61L2/18
Inventor 李晓捷罗世菊孙娟赵聚萍李霞赵楠史良赛珊梁广津王伟伟
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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