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Rapid sterilization method in routine culture of Laminaria gametophyte clone

A gametophyte, fast technology, applied in the direction of chemistry, plant cells, etc., can solve the problem of time-consuming and labor-intensive washing methods, and achieve the effect of easy operation

Active Publication Date: 2022-06-24
SHANDONG ORIENTAL OCEAN SCI TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for rapid sterilization of kelp gametophyte clones in the conventional culture process, to overcome the time-consuming and labor-intensive defects of the existing high-pressure water gun flushing method, and to realize rapid and efficient sterilization of kelp gametophyte clones

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  • Rapid sterilization method in routine culture of Laminaria gametophyte clone

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Embodiment Construction

[0016] Below in conjunction with embodiment, the present invention is further described.

[0017] 1. Selected materials: gametophyte cloning groups with severe attached bacteria. The identification method of serious attached bacteria: when the gametophyte clone surface is gray and unclean, and the clone color is not bright, further observation through the microscope shows that bacteria are attached to the surface of each branch of the gametophyte clone cluster, or the clone group is wrapped by bacteria, like figure 1 .

[0018] 2. Disinfection of the circulating pump: put the six circulating pumps into a 5000mL conical flask containing 75% alcohol at the same time, and the connecting part of the circulating pump is also immersed in the alcohol, turn on the power supply, fully disinfect for 1min, and connect the circulating pump to the connection. The lines were moved into conical flasks containing fresh water (boiled and cooled) and sea water (boiled and cooled) in turn, inf...

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Abstract

The invention relates to a rapid sterilization method in the conventional culture process of kelp gametophyte clones. Pour the gametocyte clones with serious bacterial contamination together with the culture solution into a sterilized container, then move the sterilized circulation pump into the container, and inflate and disperse the bacteria. Attach the clonal group of bacteria, then let it stand and change the water to separate and remove the bacteria. The biggest feature of the method of the invention is that it is easy to operate, fast and efficient; through the circulation pump, it kills two birds with one stone, not only removing bacteria, but also breaking up clone groups, which is beneficial to the faster growth of gametocytes.

Description

technical field [0001] The invention relates to a sterilization method for kelp gametophyte clone. Background technique [0002] Kelp is one of the important economically cultivated seaweeds in my country. With the establishment of the kelp gametophyte cloning technology and the continuous improvement of the gametophyte clone breeding technology system, the gametophyte clone breeding has become an inevitable trend of kelp breeding. Cloning seedlings can solve many disadvantages of sporophyte seedlings, and has the following technical advantages: omit the cultivation and selection of sea kelp, and reduce labor costs; Shorten seedling time and reduce refrigeration costs; because the genetic basis of gametophyte cloning is pure, it can maintain the excellent traits of the variety for a long time; the heterosis obtained by the hybridization of female and male gametophyte clonal breeding lines can be utilized through gametophyte cloning and seedling breeding. [0003] In the da...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A61L2/18A61L101/34
CPCC12N5/04A61L2/18
Inventor 李晓捷罗世菊孙娟赵聚萍李霞赵楠史良赛珊梁广津王伟伟
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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