Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mycoplasma identification agar culture medium and preparation method thereof

A technology of agar medium and culture medium, applied in the field of culture medium, can solve the problems of failing to meet the requirements of mycoplasma detection and low sensitivity, and achieve the effects of colony observation, low sensitivity and high sensitivity

Pending Publication Date: 2020-06-19
ZHUHAI ENCODE MEDICAL ENG
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as mentioned above, due to the inhibitory effect of manganese sulfate on the growth of mycoplasma, the sensitivity of this type of solid agar medium is very low, which cannot meet the requirements of mycoplasma detection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mycoplasma identification agar culture medium and preparation method thereof
  • Mycoplasma identification agar culture medium and preparation method thereof
  • Mycoplasma identification agar culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] The preparation method of above-mentioned mycoplasma identification agar medium comprises the following steps:

[0024] (1) Preparation of basal medium: Weigh agar powder, tryptone, soytone, dipotassium hydrogen phosphate, glucose, sodium chloride and yeast extract in proportion, add them directly to purified water, heat to dissolve completely, and mix thoroughly. After uniformity, adjust the pH value to 6.3±0.05 with 2mol / ml HCL, heat and boil the culture medium for 5 minutes, and then sterilize under high pressure at 121°C for 15 minutes for later use.

[0025] (2) Preparation of additives: Weigh urea, arginine, phenol red, penicillin potassium, ampicillin sodium, amphotericin, CaCl 2 , fully dissolved in purified water for later use.

[0026] (3) Preparation of culture medium: Under 100-class clean conditions, cool the basal medium after autoclaving to 55-56°C, heat the sterile-filtered serum to 55-56°C, and measure the serum according to the proportion Add to the ...

Embodiment 1

[0037] Calculated by mass percentage, the culture medium includes the following components:

[0038] Agar powder 1.0%, tryptone 1.0%, soytone 0.3%, glucose 0.3%, dipotassium hydrogen phosphate 0.3%, sodium chloride 0.3%, yeast extract 2.0%, urea 0.12%, arginine 0.12%, phenol Red 0.02%, Penicillin Potassium 0.003%, Ampicillin Sodium 0.003%, Amphotericin 0.003%, CaCl 2 0.04%, serum 5.0%, and the balance is purified water. The above ingredients were prepared for culture medium according to the above method to obtain a solid culture medium. Uu colony culture was carried out on the prepared medium, and the results shown in Table 1 were obtained.

Embodiment 2

[0040] Calculated by mass percentage, the culture medium includes the following components:

[0041] Agar powder 1.25%, tryptone 1.25%, soytone 0.45%, glucose 0.45%, dipotassium hydrogen phosphate 0.45%, sodium chloride 0.45%, yeast extract 3.0%, urea 0.19%, arginine 0.19%, phenol Red 0.035%, Penicillin Potassium 0.004%, Ampicillin Sodium 0.004%, Amphotericin 0.004%, CaCl 2 0.05%, serum 7.0%, and the balance is purified water. The above ingredients were prepared for culture medium according to the above method to obtain a solid culture medium. Uu colony culture was carried out on the prepared medium, and the results shown in Table 1 were obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a mycoplasma identification agar culture medium which is simple in components, good in bacterial colony enlarging effect and high in sensitivity and a preparation method thereof. The culture medium disclosed by the invention comprises the following components in percentage by mass: 5.2%-9.4% of a basic culture medium, 0.309%-0.645% of an additive, 5.0%-9.0% of serum and thebalance of purified water. The additive contains CaCl2, and the CaCl2 accounts for 0.04%-0.06% of the culture medium. The preparation method of the culture medium comprises the following steps: (1) preparing the basic culture medium; (2) preparing the additive; (3) preparing a culture medium; and (4) preparing a solid culture medium. The method is used in the field of culture media.

Description

technical field [0001] The invention relates to the field of medium, in particular to a mycoplasma identification agar medium for ureaplasma urealyticum and a method for culturing and identifying mycoplasma using the medium. Background technique [0002] Mycoplasma (Mycoplasma) belongs to Mollusca class, Mycoplasmales (Mycoplasmatales), Mycoplasma family, is a group of pathogens whose size is between bacteria and viruses, and is an opportunistic pathogen. Mycoplasma colonization occurs in the genitourinary tract, and there are a considerable number of mycoplasma carriers in the population without symptoms and signs, especially Uu. [0003] The culture method is currently the main method for the detection of Ureaplasma urealyticum and Mycoplasma hominis in domestic medical institutions, and it mainly uses liquid medium for direct detection and simultaneously conducts mycoplasma drug susceptibility testing. It has been used for nearly 20 years, but it has been exposed in clini...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12N1/20C12R1/35
CPCC12Q1/04C12N1/20
Inventor 孙宜峰熊伟马萍王妹曾冰冰
Owner ZHUHAI ENCODE MEDICAL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com