A kind of growth promoting agent that promotes the rapid reproduction of photosynthetic bacteria strains and its application method

A technology of photosynthetic bacteria and growth promoters, applied in the field of microorganisms, can solve the problems of unfavorable establishment of dominant populations of photosynthetic bacteria, inability to meet production applications, and small number of bacterial cells, so as to achieve large clean water or accumulate effective substances, and promote photosynthetic bacteria Effect of growth and promotion of rapid reproduction

Inactive Publication Date: 2015-11-18
HEBEI WANLIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, an excessively high pH value will inhibit the growth of photosynthetic bacteria itself, resulting in a small number of final bacterial cells; and in the process of cultivating photosynthetic bacteria with industrial and agricultural wastewater as raw material (medium), since most of the water is slightly acidic, Consuming a large amount of lye to increase the pH value is not only costly, but also pollutes the environment. In such a slightly acidic or neutral aerobic environment, the infection of miscellaneous bacteria will also be inevitable, which is not conducive to the establishment of dominant populations of photosynthetic bacteria.
In short, the existing photosynthetic bacteria production process is far from meeting the requirements of production and application in terms of the growth rate of the strain, the number of bacterial cells contained, and the purity of the bacterial cells.

Method used

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  • A kind of growth promoting agent that promotes the rapid reproduction of photosynthetic bacteria strains and its application method
  • A kind of growth promoting agent that promotes the rapid reproduction of photosynthetic bacteria strains and its application method
  • A kind of growth promoting agent that promotes the rapid reproduction of photosynthetic bacteria strains and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Follow these steps:

[0031] (1) Activation of photosynthetic bacteria (indoor):

[0032] Prepare a seed fermentation medium commonly used in the photosynthetic bacteria laboratory, put it into a transparent glass anaerobic bottle with a capacity of 150ml, and sterilize it under high pressure at 115°C for 30 minutes. After cooling, perform strict aseptic operation, insert the purchased Rhodopeudomonas palustris (Rhodopeudomonas palustris strain preservation number CGMCC1.2180) strains, and carry out continuous light culture under incandescent light at 2500 lux for 3 to 5 days, and the culture temperature is 28- At 32°C, activated photosynthetic bacteria strains were prepared. Check under the microscope that the photosynthetic bacteria are robust, active and free of pollution.

[0033] The composition of the commonly used seed fermentation medium in the laboratory is as follows:

[0034] Ammonium chloride 1.0g, sodium acetate 1.0g, magnesium sulfate 0.2g, sodium bicar...

Embodiment 2

[0050] The present invention can be operated according to the following steps:

[0051] (1) photosynthetic bacteria strain activation: with embodiment 1 (1);

[0052] (2) method is the same as embodiment 1 (2).

[0053] The difference is that the composition of the added photosynthetic bacteria growth promoter is shown in Table 3 of the design scheme,

[0054] Add different concentrations of ethanol (see the design scheme Table 3 for the amount added), and set up 7 treatments in total, numbered 1, 2, 3, 4, 5, 6, 7 (control).

[0055] table 3

[0056] serial number

1

2

3

4

5

6

7

Sorbic acid (mmol / L)

0.05

0.08

0

0

0

0

0

Potassium sorbate (mmol / L)

0

0

0.05

0.08

0

0

0

Sodium sorbate (mmol / L)

0

0

0

0

0.05

0.08

0

Ethanol (mmol / L)

0.15

0.20

0.15

0.20

0.15

0.20

0

[0057] Test results: as shown in Ta...

Embodiment 3

[0062] The present invention can be operated according to the following steps:

[0063] (1) photosynthetic bacteria strain activation: with embodiment 1 (1);

[0064] (2) Effect of photosynthetic bacteria growth-promoting agent on growth under different pH conditions (under laboratory conditions): operation method is the same as embodiment 1 (2);

[0065] The difference is that the photosynthetic bacteria growth promoter added to the commonly used seed medium in the laboratory consists of 0.05mmol / L sorbic acid and 0.2mmol / L ethanol, and the pH values ​​are adjusted to 6.5, 7.0, 7.5, 8.0 respectively .

[0066] Test results: Table 5 shows the culture results of culture conditions with different pH values.

[0067] table 5

[0068] pH value

[0069] The test results showed that within the pH range of 6.5-8.0, adding 0.05mmol / L sorbic acid and 9mmol / L ethanol could promote the growth of photosynthetic bacteria to varying degrees, and after 4 days of cultivation, the...

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Abstract

The invention relates to a growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and a using method of the growth promoting agent. The growth prompting agent is obtained as follows: adding 1 part weight of one or a mixture of sorbic acid, potassium sorbate or sodium sorbate to 1-4 parts by weight of alcohol for stirring, mixing and dissolving. The using method of the growth promoting agent is as follows: adding the growth promoting agent to a prepared photosynthetic bacteria liquid medium for stirring until the growth promoting agent is sufficiently dissolved; adding active photosynthetic bacteria strains for filling to a sealed or semi-open light transmission container for culturing at 25-32 DEG C by radiating under 1500lux-1600lux, wherein sorbate radial concentration of the growth promoting agent, which is added to the photosynthetic bacteria liquid medium, is controlled within a range of 0.005 mmol / L-1.0 mmol / L. The growth promoting agent for promoting photosynthetic bacteria strain to quickly breed is simple to use, less in usage, low in cost and capable of promoting photosynthetic bacteria strain to quickly breed. Moreover, the using method of the growth promoting agent is safe, ecological and environment-friendly and extensive in market prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a growth-promoting agent for promoting the rapid propagation of photosynthetic bacteria strains and a use method thereof. Background technique [0002] Photosynthetic bacteria (Photosynthetic Bacteria referred to as PSB) ecological distribution is extremely wide, mainly distributed in oceans, rivers, lakes, activated sludge and soil, is a type of aquatic, hydrosphere microorganisms. Photosynthetic bacteria use sunlight as energy source, sodium acetate, sodium lactate, mannitol, malic acid, citric acid, glucose, glycerin and other organic substances as carbon and nitrogen sources, with fast growth and reproduction speed, wide source of medium, metabolite nutrition rich features. Photosynthetic bacterial cells are rich in protein, amino acid, biologically essential vitamins, antiviral active factors, coenzyme Q10 and a variety of physiologically active substances, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 张根伟张闻笛王子晴
Owner HEBEI WANLIN BIOTECH
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