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An in vitro detection kit for anaerobic microorganisms

An in vitro detection technology for anaerobic microorganisms, which is applied in the field of in vitro detection kits for anaerobic microorganisms, can solve the problems of short detection rate, detection time, unclear effect, and difficulty in judging the actual effect of blood culture bottles, so as to reduce the impact , Improve the effect of bacterial flora activity

Active Publication Date: 2022-03-18
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patent CN104450506B discloses a preparation method of anaerobic blood culture medium, culture bottle and culture bottle. In the invention, two substances, glucose oxidase and peroxidase, are added to the medium, which can remove the medium Oxygen in the medium and hydrogen peroxide and other cytotoxic substances that are toxic to bacteria produced during the metabolic process solve the problems of low positive detection rate, complicated production and high cost in traditional methods
However, the invention does not specify the specific type of anaerobic bacteria, so it is not known whether the blood culture bottle is universal for the detection of anaerobic bacteria. At the same time, the detection rate and detection time are not specified, so it is difficult to judge The actual effect of this blood culture bottle
[0005] Aiming at the problems of unclear effect and universal applicability of the existing technology, it is necessary to find an in vitro detection kit for anaerobic microorganisms, so that the kit can be generally applied to the detection of various anaerobic bacteria, and has a relatively High detection rate and short detection time

Method used

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  • An in vitro detection kit for anaerobic microorganisms
  • An in vitro detection kit for anaerobic microorganisms
  • An in vitro detection kit for anaerobic microorganisms

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Experimental program
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Effect test

Embodiment 1

[0025] An in vitro detection kit for anaerobic microorganisms, characterized in that it includes a culture solution, an adsorption resin, a culture bottle body, a bottle cap and a sensor;

[0026] Among them, the culture solution includes 1g / L tryptone, 1g / L gelatin peptone, 1g / L brain heart infusion, 1g / L L-arginine, 1g / L L-cysteine ​​hydrochloride, 0.08g / L L activity synergistic regulator, 0.2g / L sodium citrate, 0.2g / L potassium dihydrogen phosphate and 0.8g / L vitamin C. The active synergistic regulator comprises sitosterol and hemin in a weight ratio of 1:3. The above culture solution was prepared with double distilled water.

[0027] In addition, the adsorption resin is a mixture of anion exchange resin and macroporous adsorption resin with a volume ratio of 1:1, and the sensor is located at the bottom of the culture bottle and contains a fluorescent dye.

Embodiment 2

[0029] An in vitro detection kit for anaerobic microorganisms, characterized in that it includes a culture solution, an adsorption resin, a culture bottle body, a bottle cap and a sensor;

[0030] Among them, the culture solution includes 10g / L tryptone, 10g / L gelatin peptone, 10g / L brain heart infusion, 10g / L L-arginine, 10g / L L-cysteine ​​hydrochloride, 0.3g / L L activity synergistic regulator, 0.5g / L sodium citrate, 0.5g / L potassium dihydrogen phosphate and 2g / L vitamin C. The active synergistic regulator comprises stigmasterol and hemin in a weight ratio of 2:5. The above culture solution was prepared with double distilled water.

[0031] In addition, the adsorption resin is a mixture of anion exchange resin and macroporous adsorption resin with a volume ratio of 1:1, and the sensor is located at the bottom of the culture bottle and contains a fluorescent dye.

Embodiment 3

[0033] An in vitro detection kit for anaerobic microorganisms, characterized in that it includes a culture solution, an adsorption resin, a culture bottle body, a bottle cap and a sensor;

[0034] Among them, the culture solution includes 5g / L tryptone, 5g / L gelatin peptone, 5g / L brain heart infusion, 3.8g / L L-arginine, 2.4g / L L-cysteine ​​hydrochloride, 0.2 g / L active synergistic regulator, 0.3g / L sodium citrate, 0.3g / L potassium dihydrogen phosphate and 1.2g / L vitamin C. The active synergistic regulator comprises campesterol and hemin in a weight ratio of 1:4. The above culture solution was prepared with double distilled water.

[0035] In addition, the adsorption resin is a mixture of anion exchange resin and macroporous adsorption resin with a volume ratio of 1:1, and the sensor is located at the bottom of the culture bottle and contains a fluorescent dye.

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Abstract

The invention provides an in vitro detection kit for anaerobic microorganisms, which relates to the technical field of biochemical detection. The kit includes a culture solution, an adsorption resin, a culture bottle body, a bottle cap and a receptor; the culture solution includes tryptone, Gelatin peptone, brain heart infusion, L-arginine, L-cysteine ​​hydrochloride, active synergist, sodium citrate, monopotassium phosphate and vitamin C. The kit can effectively neutralize antibiotics and provide more favorable growth conditions for microorganisms, thereby increasing the positive detection rate of anaerobic microorganisms and shortening the detection time.

Description

technical field [0001] The invention relates to the technical field of biochemical detection, in particular to an in vitro detection kit for anaerobic microorganisms. Background technique [0002] Bacteremia means that external bacteria enter the blood system through the entrance of the body surface or infected wounds, multiply in the human blood and spread throughout the body along with the blood flow. Bacteremia is mostly caused by bacteria entering the blood from local lesions, and the whole body has no symptoms of poisoning, but bacteria can be found in the blood, mainly in the early stage of inflammation. Sepsis refers to the acute systemic infection caused by pathogenic bacteria or opportunistic pathogenic bacteria invading the blood circulation, growing and multiplying in the blood, and producing toxins. Because most sepsis is secondary to various infections and lacks specific clinical manifestations, it is easy to cause missed or misdiagnosed cases. Blood cultures ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12Q1/10C12Q1/14C12R1/145C12R1/46C12R1/19C12R1/445C12R1/01
Inventor 娄斌章晓庆
Owner 浙江夸克生物科技有限公司
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