Application of pyridyl ancymidol compounds in plant induced resistance
A technology of pyridyl pyrimidinol and induction of resistance, which is applied in the fields of application, plant growth regulator, plant growth regulator, etc., and can solve the problems of affecting plant growth and poor water solubility
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Embodiment 1
[0028] Embodiment 1 Experimental material and method
[0029] 1. Plant materials and strains
[0030] Plant material: Arabidopsis thaliana wild type (Columbia, Col); rice (Oryza Sativa).
[0031] Strain: Botrytis cinerea, NJ-09 strain provided by China Agricultural University, Pseudomonas syringe.DG3, the above strains provided by Jean Laboratory, University of Chicago, USA.
[0032] 2. Main experimental reagents
[0033] Trypan blue and DAB drugs were purchased from Guangzhou Weijia Technology Co., Ltd. (Sigma imported repackaging); plant activator BTH was purchased from Japan Wako Company; plant activator RJF was purchased from British Maybridge Company; common reagents were analyzed by Guangzhou Chemical Reagent Factory Pure reagents.
[0034] 3. Preparation of nutrient solution and important reagents used in the experiment
[0035] 3.1 The formula of B5 nutrient solution
[0036] Solution A(100×)
[0037] 300mg H 3 BO 3
[0038] 100mg MnSO 4 ·H 2 o
[0039] 200...
Embodiment 2
[0122] Embodiment 2 The effect of novel plant activator of the present invention in the interaction between Arabidopsis thaliana and pathogenic bacteria
[0123] 1. Effects of different concentrations of RJF on the growth and development of Arabidopsis thaliana seedlings
[0124] Spread the seeds of Arabidopsis wild-type (Col) evenly on the surface of the 1 / 2MS medium containing the plant activator, put them into the plant room for cultivation, and observe the phenomenon.
[0125] Such as figure 1 , Arabidopsis grown under different concentrations did not appear to inhibit growth.
[0126] 2. Effects of BTH and RJF on root growth and development of Arabidopsis seedlings alone
[0127] Such as figure 2 A, From the plate growth experiment of Arabidopsis, it can be seen that in the 12-day BTH treatment group, the growth of Arabidopsis was inhibited, and the seedlings were short; while in the 12-day RJF treatment group, the growth of the seedlings was not affected It can be s...
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