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Method for controlling contaminated bacteria in large-scale cultivation of Chlorella vulgaris and preparation

A technology of chlorella and polluting bacteria, applied in the biological field, can solve problems such as fungicides and antibiotics polluting the environment, restricting the industrial production of chlorella, and affecting the protein quality of algae cells, so as to increase anti-pollution performance and continuous antibacterial ability , The effect of removing miscellaneous bacteria

Active Publication Date: 2020-04-07
GUANGZHOU LYUBAIDUO BIOLOGICAL DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both physical and chemical methods have caused a series of problems such as ineffective use, bactericides and antibiotics polluting the environment, and affecting the protein quality of algae cells. So far, there has not been a good solution to the bacterial contamination of chlorella culture. Scientific Research Difficulties in Algae Industrial Production
There has not been any report on the method of natural plant product control method or biological agent to control the contamination of bacteria

Method used

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  • Method for controlling contaminated bacteria in large-scale cultivation of Chlorella vulgaris and preparation
  • Method for controlling contaminated bacteria in large-scale cultivation of Chlorella vulgaris and preparation
  • Method for controlling contaminated bacteria in large-scale cultivation of Chlorella vulgaris and preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Under the condition that no treatment liquid was added during the cultivation of Chlorella, the cell concentration of Chlorella ellipsoids changed from 6.0×10 after 13 days of cultivation 6 cells / mL increased to 3.6×10 7 pcs / mL (refer to attached figure 1 ), at the same time the cell concentration of contaminating bacteria in the Chlorella ellipsoid culture increased from the initial 5.6×10 6 cells / mL increased to 7.8×10 7 cells / mL (refer to Figure 2). This result indicated that during the cultivation process of chlorella, along with the growth of chlorella, the contaminating bacteria also grew further.

[0031] Contaminating bacteria for cell counts include Gram-negative bacteria, Gram-positive bacteria, and Escherichia coli.

Embodiment 2

[0033] Add the prepared Polygonum cuspidatum extract and Agarwood extract during the cultivation of Chlorella, wherein the raw materials of the Polygonum cuspidatum extract and Agarwood extract are specially treated as described in the instructions, and the weight-number ratio of the Polygonum cuspidatum extract to the Agarwood extract is 1 :10, the weight ratio of the total weight of Polygonum cuspidatum extract and Agarwood extract to the culture system of Chlorella is 1:30; after cultivating for 13 days, the cell concentration of Chlorella ellipsoids is from 6.0×10 6 cells / mL increased to 4.8×10 8 pcs / mL (refer to attached figure 1 ), at the same time the cell concentration of contaminating bacteria in the Chlorella ellipsoid culture increased from the initial 5.6×10 6 cells / mL decreased to 4.8×10 4 pcs / mL (refer to attached figure 2 ). This result indicated that during the cultivation process of Chlorella, along with the growth of Chlorella, the contaminating miscella...

Embodiment 3

[0037] The existing bactericide mancozeb was added during the cultivation of Chlorella, and after 11 days of cultivation, the cell concentration of Chlorella ellipsoids increased from 6.0×10 6 cells / mL increased to 5.2×10 7 cells / mL, at the same time, the cell concentration of contaminating bacteria in the Chlorella ellipsoid culture changed from the initial 5.6×10 6 cells / mL decreased to 6.2×10 on the third day 5 individual / mL, and then began to increase and reached a concentration of 3.6×10 on the 11th day 7 pcs / mL (refer to attached image 3 ). This result shows that during the cultivation of chlorella in Example 3, along with the growth of chlorella, the contaminating miscellaneous bacteria were initially suppressed, but then the concentration of contaminating miscellaneous bacteria rebounded.

[0038] From the test data of Examples 1-3, it can be concluded that the method for controlling polluting bacteria of the present invention has obvious advantages compared with ...

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Abstract

The invention provides a method for controlling contaminated bacteria in large-scale cultivation of Chlorella vulgaris. The method is to control and / or reduce the number of other contaminated miscellaneous bacteria by adding a plant extract into a Chlorella vulgaris cultivation system, thus promoting the growth of Chlorella vulgaris. The plant extract comprises a Polygonum cuspidatum extract and achinese eaglewood extracting solution, and the weight ratio of the Polygonum cuspidatum extract to the chinese eaglewood extracting solution is 1: 8-20. By adopting the Polygonum cuspidatum extract and the chinese eaglewood extracting solution in the Chlorella vulgaris cultivation system, not only the miscellaneous bacteria are removed, but also the anti-pollution performance of Chlorella vulgaris is increased. The Polygonum cuspidatum extract has a promoting effect on the growth and antibacterial property of Chlorella vulgaris, and the chinese eaglewood extracting solution has no inhibitoryeffect on the growth of Chlorella vulgaris.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for controlling other polluting bacteria in large-scale cultivation of chlorella by using a biological method, thereby promoting the growth of chlorella. Background technique [0002] As one of the simplest photosynthetic organisms, Chlorella is the earliest microalgae that can be cultivated by humans. It not only grows fast, but also is rich in protein, polysaccharides, vitamins and Chlorella growth factors and other nutrients and biologically active components. It has received widespread attention at home and abroad, and has been widely used in human health food, bioenergy and environmental governance. [0003] Generally speaking, there are three ways of cultivating Chlorella: photoautotrophic, polyculture and non-light heterotrophic culture, but there are inevitably different degrees of bacterial contamination in both laboratory and industrial large-scale cultivation. Beca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38A01N65/30A01N65/08A01N31/16A01N31/04A01N43/16A01P1/00C12R1/89
CPCA01N31/04A01N31/16A01N43/16A01N65/00A01N65/08A01N65/30C12N1/12C12N1/38
Inventor 张金燕徐中文翟彦军刘巧林
Owner GUANGZHOU LYUBAIDUO BIOLOGICAL DEV CO LTD
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