Method for promoting rooting of butterfly orchid tissue cultured seedling by utilizing LED light source

An LED light source and phalaenopsis technology, which is applied in the field of plant tissue culture, can solve the problems of not giving the light-quality ratio, not involving the influence of far-red light on phalaenopsis, etc., so as to increase the number of roots, improve the quality of seedlings, and improve dry matter. the cumulative effect of

Active Publication Date: 2015-03-25
ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

Hsu[Hsing-cheng Hsu,Chiachung Chen(2010).The effect of light spectrum on the growth characteristics of in vitro cultures of phalaenopsis.Propagation of Ornamental Plants 10,3-8.] and Dai Yanjiao[Dai Yanjiao,Wang Qiongli,Zhang Huan, Dai Dayong, Wen Mincao, Xu Zhigang (2010). Effects of LEDs with different spectra on the growth of Phalaenopsis tissue culture seedlings. Jiangsu Agricultural Science 5, 227-231.] Research on the influence of LEDs on Phalaenopsis rooting, but the light quality The comparison is only limited to the influence of monochromatic red light, monochromatic blue light and unknown ratio of red and blue combination on the rooting of Phalaenopsis. The most suitable light quality ratio has not been given, and the effect of far-red light on Phalaenopsis tissue culture has not been involved. influences

Method used

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  • Method for promoting rooting of butterfly orchid tissue cultured seedling by utilizing LED light source
  • Method for promoting rooting of butterfly orchid tissue cultured seedling by utilizing LED light source
  • Method for promoting rooting of butterfly orchid tissue cultured seedling by utilizing LED light source

Examples

Experimental program
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Effect test

Embodiment 1

[0045] (1) In the experiment, the flower stalks of Phalaenopsis 'Green Bear' (Green Bear) purchased from the Jinying Flower Seedling Promotion Center of the Institute of Environmental Horticulture, Guangdong Academy of Agricultural Sciences were selected as explants. Differentiation was induced on the pedicel axillary bud induction medium, and the induction culture conditions were culture temperature 25±2°C, relative humidity 50%-60%, light time 12h / d, and cultured in the ordinary tissue culture light environment of the covering newspaper in the previous week. When the light intensity is 5±1μmol·m -2 ·s -1 Cultivate for 7 days in the normal tissue culture light environment, after uncovering the newspaper, continue to culture in the normal tissue culture light environment for 45-60 days, that is, when the light intensity is 20±2μmol·m -2 ·s -1 Cultivate for 45-60 days under ordinary tissue culture light environment to obtain clustered buds;

[0046](2) Cut the clustered buds...

Embodiment 2

[0064] What this embodiment is different from Example 1 is that the experimental material that adopts is that the large flower line Phalaenopsis 'big Chilli' (Big Chilli) purchased from the Guangdong Academy of Agricultural Sciences Environmental Horticulture Seedlings Promotion Center is a material, and the rest are the same as Example 1 is the same.

[0065] The effect of LEDs with different light qualities on the rooting production of 'big pepper' is as follows:

[0066] Under the treatment of four LEDs, the root length of 'Big Pepper' was significantly increased compared with the control (WFL) (Table 4), and the values ​​of the two treatments (R6B3FR1, R3B6FR1) added with far-red light were higher, respectively increased by 59.56 compared with the control % and 64.16%. The number of roots was also higher under the two treatments (R6B3FR1, R3B6FR1) added with far-red light, and significantly increased compared with the control, which increased by 39.02% and 35.61%, respect...

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Abstract

The invention belongs to the field of plant tissue culture, and particularly relates to a method for promoting rooting of a butterfly orchid tissue cultured seedling by utilizing an LED light source. The method specifically comprises the following steps: (1) performing induced culture by taking a butterfly orchid flower as an explant to obtain a cluster bud; (2) multiplying and culturing the cluster bud obtained in the step (1), dividing the multiplied and cultured cluster buds into single buds and inoculating the single buds into a rooting culture medium; (3) culturing and rooting the cluster buds which are inoculated into the rooting culture medium in mixed light consisting of three single-color lights including red light, blue light and far-red light. According to the method, the treatment of the mixed light added with the far-red light promotes the growth of the roots of butterfly orchid seedlings, so that accumulation of dry matters is increased, and the growth of the underground parts is not inhibited; meanwhile, the method has the advantages of lowering the production cost and saving energy.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for promoting rooting of moth orchid tissue culture seedlings by utilizing LED light sources. Background technique [0002] Phalaenopsis (Phalaenopsis ssp.) is a plant of the genus Phalaenopsis in the family Orchidaceae. It has a unique flower shape, elegant posture, and long flowering period. It is known as the "Queen of Orchids" and occupies an important position in the flower market at home and abroad. . [0003] The widely used Phalaenopsis propagation method in production is tissue culture technology. Its commonly used approach is cluster bud approach, including several stages of explant induction cluster bud, cluster bud proliferation, rooting and strong seedlings, wherein the growth state of seedlings in the rooting and strong seedling stage is very important for the growth of seedlings after transplanting. influences. The traditional tissue cult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/04
CPCY02P60/14
Inventor 朱根发任桂萍刘海林
Owner ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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