A Bacillus pumilus strain and its application
A technology of Bacillus pumilus and strains, applied in the field of microbial engineering, can solve the problems of slow fertilizer efficiency, insufficient use of nutrients, and waste of resources.
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[0032] A strain of Bacillus pumilus provided by the present invention, which is called Bacillus pumilus CFC-5 (Bacillus pumilus CFC-5), is preserved in the Guangdong Microbial Culture Collection Center (abbreviated as GDMCC), and it is stored in the Guangdong Provincial Microbial Culture Collection Center. The preservation number is GDMCC No: 60397, and the preservation time is June 25, 2018. The address of the preservation unit is located at Guangdong Institute of Microbiology, 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City.
[0033] 1. Screening of bacterial strains
[0034] 1) Source of tung oil cake: sampling from oil tung processing enterprises.
[0035] 2) Tung cake extract juice medium: tung cake cake 200g, soaked at 60-90°C for 3-4 hours, filtered through 8 layers of gauze to remove the residue to obtain tung meal juice, set the volume to 1000mL, and packed in 250mL triangular flasks. The bottled volume is 50mL, and the pH is natural.
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