A Bacillus pumilus strain and its application

A technology of Bacillus pumilus and strains, applied in the field of microbial engineering, can solve the problems of slow fertilizer efficiency, insufficient use of nutrients, and waste of resources.

Active Publication Date: 2021-06-22
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tung cake cake is used as fertilizer directly or after accumulation and fermentation, not only the fertilizer effect is slow, but also the nutrients in the tung cake can not be fully utilized, resulting in waste of resources

Method used

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  • A Bacillus pumilus strain and its application
  • A Bacillus pumilus strain and its application
  • A Bacillus pumilus strain and its application

Examples

Experimental program
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Effect test

Embodiment

[0032] A strain of Bacillus pumilus provided by the present invention, which is called Bacillus pumilus CFC-5 (Bacillus pumilus CFC-5), is preserved in the Guangdong Microbial Culture Collection Center (abbreviated as GDMCC), and it is stored in the Guangdong Provincial Microbial Culture Collection Center. The preservation number is GDMCC No: 60397, and the preservation time is June 25, 2018. The address of the preservation unit is located at Guangdong Institute of Microbiology, 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City.

[0033] 1. Screening of bacterial strains

[0034] 1) Source of tung oil cake: sampling from oil tung processing enterprises.

[0035] 2) Tung cake extract juice medium: tung cake cake 200g, soaked at 60-90°C for 3-4 hours, filtered through 8 layers of gauze to remove the residue to obtain tung meal juice, set the volume to 1000mL, and packed in 250mL triangular flasks. The bottled volume is 50mL, and the pH is natural.

[...

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Abstract

The invention discloses a strain of Bacillus pumilus, which is preserved in the Guangdong Provincial Microbial Culture Collection Center, named as Bacillus pumilus CFC‑5 (Bacillus pumilus CFC‑5), which is located in Guangdong The preservation number of the Provincial Microbial Culture Collection Center is GDMCC No: 60397, and the preservation time is June 25, 2018. The Bacillus pumilus strain provided by the present invention is Bacillus pumilus CFC‑5, which has the ability to degrade the protein in the tung tree cake, has a broad-spectrum inhibitory effect on soil-borne diseases of crops, and has a growth-promoting effect. The meal provides a source of bacteria. The invention also discloses the application of the bacterial strain in degrading protein in tung oil cake and inhibiting crop diseases.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a strain of Bacillus pumilus and its application. Background technique [0002] Tung tung is a native woody oil tree species in my country, which has the advantages of fast growth, high yield, wide adaptability, high seed oil content and rich nutrition. The tung cake after tung seed oil extraction contains 4% to 6% nitrogen, P 2 o 5 1.8%~2.7%, K 2 O 1.2% ~ 1.3%, and a large number of other trace elements, is an excellent protein resource. However, because tung tree cake contains toxins such as saponin, phorbol and related chemical substances, tung tree cake has been used as cake fertilizer for a long time and cannot be used as a source of animal food. [0003] In agricultural production, the excessive use of high-nutrient and convenient chemical fertilizers has brought about various agricultural and environmental problems: soil nutrient loss, environ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C05F15/00C05F17/20C12R1/07
CPCA01N63/00C05F5/002C05F17/00C12N1/205C12R2001/07C05F11/08Y02W30/40
Inventor 曾超珍黎继烈彭映辉吴耀辉王卫方勤敏任泽文
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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