Glucose stimulated insulin secretion determination model

A technology for insulin secretion and glucose, applied in the medical field, can solve the problems of limited curative effect, toxic and side effects, etc.

Inactive Publication Date: 2018-12-18
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Insulin secretion-stimulating drugs are mainly sulfonylureas and glinides that target sulfonylurea receptors, but there are problems such as limited curative effect and easy to produce toxic side effects

Method used

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  • Glucose stimulated insulin secretion determination model
  • Glucose stimulated insulin secretion determination model
  • Glucose stimulated insulin secretion determination model

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The present invention will be described in detail below in combination with specific embodiments.

[0023] 1 Optimization of cell density and determination of administration time

[0024] Since cell density has a great influence on cell growth state and growth trend, the density of islet β cells seeded in 96-well plate (according to the pre-experimental results, set 2000 / well, 4000 / well, 6000 / well , 8000 cells / well and other 4 gradient densities, n=3) were optimized, and the islet β cells were investigated from two aspects of cell growth state and growth trend.

[0025] The result is as figure 1 with figure 2 As shown, in the 96-well plate, the cell density ranges from 2000 cells / well, 4000 cells / well, 6000 cells / well to 8000 cells / well. Well, 8000 / well has already taken place to some degree of confluence ( figure 1 ); From the cell growth curve, the cells with a density of 8000 cells / well grew faster, and when the culture time was from 16h to 24h, the growth rate ...

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Abstract

The invention discloses a glucose stimulated insulin secretion determination model. 24 hours are determined as culture and drug administration time of pancreatic beta cells; insulin concentration is determined by utilizing an ELISA method; an OD value is determined by virtue of a CCK8 method, thus quantity of the cells is indirectly reflected; HBSS solution is selected and used as later extracellular buffer solution; and an insulin secretion positive drug glibenclamide is selected for promoting insulin secretion of the pancreatic beta cells. The model built in the invention can reflect difference between different stimuli, namely high glucose and basic glucose, and can reflect insulin secretion stimulating effects of the positive drug glibenclamide under the high glucose and basic glucoseconditions.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a glucose-stimulated insulin secretion measurement model. Background technique [0002] Type 2 diabetes is the main type of diabetes, accounting for 90% to 95%. It is a chronic hyperglycemic disease caused by insulin resistance with insufficient insulin and insulin secretion defects with or without insulin resistance. The occurrence of type 2 diabetes is closely related to the production of insulin by pancreatic β cells, the transport of insulin by the blood circulation system, and the acceptance of insulin by target cells and their physiological effects. These three processes are the basis for the development of type 2 diabetes. Important types of type 2 diabetes drug development. Insulin secretion-stimulating drugs are mainly sulfonylureas and glinides that target sulfonylurea receptors, but there are problems such as limited curative effect and easy to produce toxic side effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5008
Inventor 张从晓王克威刘慧芳
Owner QINGDAO UNIV
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