Application of molecular marker and its polymorphism in identification of branching number traits in soybean

Abstract

The present invention discloses the application of a soybean molecular marker and its polymorphism in the identification of branching number traits in soybean. The soybean molecular marker disclosed by the invention is a DNA molecule obtained by amplifying BRCH with a soybean genomic DNA as a template and a primer. BRCH consists of single-stranded DNA named BRCH_F and BRCH_R, BRCH_F is a single-stranded DNA that specifically binds to the 84th upstream of SEQ ID NO: 1 in soybean genomic DNA, and BRCH_R is a single-stranded DNA that specifically binds to the 84th downstream of SEQ ID NO: 1 in soybean genomic DNA. Experimental proof, Among the genotypes distinguished by the soybean molecular markers according to the present invention, the number of branches of the GG genotype soybean is significantly higher than that of the TT genotype soybean, indicating that the soybean molecular markers of the present invention are closely related to the number of branches of the soybean, can be used for identifying the number of branches of the soybean, and can also be used for soybean breeding according to the molecular markers of the soybean.

Description

technical field [0001] The invention relates to the application of soybean molecular markers and polymorphisms in the identification of soybean branch number traits in the field of biotechnology. Background technique [0002] Soybean is an important food and economic crop in the world, an important source of vegetable fat and protein for human beings, and occupies an important position in the daily diet structure. However, in the past ten years, my country's total soybean output has continued to decline, and my country's soybean dependence on foreign countries has continued to increase. In 2016, my country imported 83.91 million tons of soybeans, an increase of 2.7% compared with 2015 (81.69 million tons), setting a record high and reaching about 7 times the domestic soybean production. The main reason for the tight supply and demand of soybean in my country is the low yield per unit area, so increasing soybean yield per unit area has always been an important goal of soybea...

AI Technical Summary

Problems solved by technology

However, due to the complexity of the soybean genome, there are a large number of repetitive sequences; the number of branches is a quantitative trait controlled by multiple genes, and the genetic structure is complex, so the research on soybean branching traits is still in its infancy, and there is no map-based cloning of new genes. to report

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