Application of molecular marker and its polymorphism in identification of branching number traits in soybean

A technology of molecular markers and branch number, applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of complex genetic structure, complex soybean genome, which is still in its infancy

Active Publication Date: 2019-01-18
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, due to the complexity of the soybean genome, there are a large number of repetitive sequences; the number of branches is a quantitative trait controlled by multi...

Method used

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  • Application of molecular marker and its polymorphism in identification of branching number traits in soybean
  • Application of molecular marker and its polymorphism in identification of branching number traits in soybean
  • Application of molecular marker and its polymorphism in identification of branching number traits in soybean

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Embodiment 1

[0041] Example 1. Identification of soybean branch number traits using soybean molecular markers

[0042] The soybean molecular marker provided in this example is a DNA molecule obtained by using soybean genomic DNA as a template and using primers to amplify BRCH. The applicant of the present invention has found that soybean molecular markers have two sequences: sequence 1 and sequence 2. The difference between sequence 1 and sequence 2 is that the 84th position of sequence 1 is T, the 84th position of sequence 2 is G, and the other sequences are all same.

[0043] Among them, BRCH is composed of single-stranded DNA named BRCH_F and BRCH_R, BRCH_F is the single-stranded DNA shown in the 1-20th position of sequence 1, and BRCH_R is the reverse direction of the single-stranded DNA shown in the 173-197th position of sequence 1 Complementary single-stranded DNA.

[0044]The genotype of the soybean whose PCR product sequence of the primer pair BRCH is sequence 1 is marked as TT g...

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Abstract

The present invention discloses the application of a soybean molecular marker and its polymorphism in the identification of branching number traits in soybean. The soybean molecular marker disclosed by the invention is a DNA molecule obtained by amplifying BRCH with a soybean genomic DNA as a template and a primer. BRCH consists of single-stranded DNA named BRCH_F and BRCH_R, BRCH_F is a single-stranded DNA that specifically binds to the 84th upstream of SEQ ID NO: 1 in soybean genomic DNA, and BRCH_R is a single-stranded DNA that specifically binds to the 84th downstream of SEQ ID NO: 1 in soybean genomic DNA. Experimental proof, Among the genotypes distinguished by the soybean molecular markers according to the present invention, the number of branches of the GG genotype soybean is significantly higher than that of the TT genotype soybean, indicating that the soybean molecular markers of the present invention are closely related to the number of branches of the soybean, can be used for identifying the number of branches of the soybean, and can also be used for soybean breeding according to the molecular markers of the soybean.

Description

technical field [0001] The invention relates to the application of soybean molecular markers and polymorphisms in the identification of soybean branch number traits in the field of biotechnology. Background technique [0002] Soybean is an important food and economic crop in the world, an important source of vegetable fat and protein for human beings, and occupies an important position in the daily diet structure. However, in the past ten years, my country's total soybean output has continued to decline, and my country's soybean dependence on foreign countries has continued to increase. In 2016, my country imported 83.91 million tons of soybeans, an increase of 2.7% compared with 2015 (81.69 million tons), setting a record high and reaching about 7 times the domestic soybean production. The main reason for the tight supply and demand of soybean in my country is the low yield per unit area, so increasing soybean yield per unit area has always been an important goal of soybea...

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 邱丽娟郭勇张霞张勇杨兴勇
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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