Composition containing stem cell active substance and preparation method thereof

A technology of active substances and compositions, applied in the field of kits and cells and cell culture, which can solve the problems of cell lethality and achieve the effects of reducing damage, enhancing effect, and reducing damage

Active Publication Date: 2020-11-10
厦门神雁再生医学科技有限公司
View PDF16 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] However, in the aforementioned prior art, the great difficulty encountered during cryopreservation of cells is the cell lethality of the freezing and thawing (i.e., freeze-thaw) process, which poses a great challenge to the stem cell cryopreservation protocol and business

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition containing stem cell active substance and preparation method thereof
  • Composition containing stem cell active substance and preparation method thereof
  • Composition containing stem cell active substance and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Take fresh human-derived adipose-derived mesenchymal stem cells (obtained from Shandong Qilu Stem Cell Engineering Co., Ltd.), the micrographs of their morphology are as follows figure 1 Shown. The stem cells were added to a cryoprotectant composed of 1.0 wt.% DMSO (cell culture medium, purchased from Sigma), 2.0 wt.% glutathione (from Beijing Biolab Company) , 2.0 wt.% trehalose (from Sinopharm Chemical Reagent Co., Ltd.) and 95 wt.% fetal bovine serum (FBS) (from Shanghai Yubo Biotechnology Co., Ltd.), the stem cells are 5×10 6 One, the cryoprotectant is 2.0 ml, and it is stored frozen for 15 months. After the storage period is over, use conventional thawing method to thaw, observe its morphology with a microscope, and its morphology is like figure 2 Shown.

[0071] It was observed that after cryopreservation of stem cells using the cryoprotectant of the present invention, even after 15 months of preservation, they can obtain fibroblast-like shapes similar to fresh st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a composition containing stem cell active substances and a preparation method thereof. The composition contains stem cells and a culture medium, and the culture medium is a low-serum medium or a serum-free medium. Kits comprising the compositions are also disclosed. The composition containing stem cell active substances has high freeze-thaw reliability.

Description

Technical field [0001] The present invention belongs to the technical field of kits and cell and cell culture. It relates to a composition containing stem cell active substances and a preparation method thereof, and in particular to a composition containing stem cell active substances, a kit containing the composition and a preparation method thereof. Background technique [0002] Stem cells have the potential for proliferation and differentiation. They are capable of self-renewal and replication and can produce highly differentiated functional cells. In other words, they are a type of primitive undifferentiated cells with multidirectional differentiation potential and self-replication ability, forming mammals. Primitive cells of various tissues and organs of animals. [0003] Due to the characteristics of stem cells, they have a wide range of applications. At present, stem cells have been used to prepare reagents or kits that can be applied to various diseases, or to prepare healt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0221C12N5/0667C12N2500/90A01N1/0226C12N2500/44C12N2500/32C12N2501/125C12N2501/115C12N2501/135C12N2501/15
Inventor周勇王全立陈海旭耿培亮
Owner厦门神雁再生医学科技有限公司