Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Eye drops containing canine mesenchymal stem cell factor and preparation method thereof

A technology of stem cells and eye drops, applied in the field of veterinary medicine, can solve the problems of lack of very effective treatment plan and impaired vision, and achieve the effect of speeding up the healing speed, promoting the repair of corneal damage, and being beneficial to large-scale industrial production.

Active Publication Date: 2022-05-10
维赛(佛山)生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Canine corneal injury accounts for at least half of the serious eye injuries in dogs. If the canine corneal injury is not treated and repaired in time, the scar tissue formed will lead to greatly weakened vision. However, there is no very effective treatment for dog corneal injury repair at present Program

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Eye drops containing canine mesenchymal stem cell factor and preparation method thereof
  • Eye drops containing canine mesenchymal stem cell factor and preparation method thereof
  • Eye drops containing canine mesenchymal stem cell factor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] An eye drop containing canine mesenchymal stem cell factor, which consists of canine adipose mesenchymal stem cell culture supernatant 48%, chloramphenicol 0.2%, sodium hyaluronate 0.45%, polyethylene glycol 400 5%, 1,2-propanediol 3.5% and the balance of normal saline.

[0024] Preparation:

[0025] 1) Take about 3ml of dog abdominal fat, wash it with PBS containing 10% double antibody, use sterilized ophthalmic scissors to cut the fat block to 1mm size, use type Ⅰ collagenase to enzymolyze it in a 37°C incubator for 40min, and sterilize Sieve through the bacterial single-cell filter membrane, centrifuge at 1200r / min for 5min to remove the supernatant, resuspend the cells with complete medium, add an appropriate amount of complete medium to a petri dish, and culture in a 5% carbon dioxide incubator at 37°C. Cells grow to a density of about 80% and pass down to 3-6 passages for identification. Take the culture supernatant and centrifuge it at 4000r / min for 20min, filte...

Embodiment 2

[0029] An eye drop containing canine mesenchymal stem cell factor, which consists of canine placental mesenchymal stem cell culture supernatant 40%, chloramphenicol 0.28%, sodium hyaluronate 0.25%, polyethylene glycol 600 8%, 1,2-propanediol 1.5% and the balance of normal saline.

[0030] As a further improvement of the above scheme, the supernatant of the canine mesenchymal stem cell culture medium is obtained by collecting the supernatant of canine mesenchymal stem cells through cell culture, centrifuging at 4000r / min for 20min, and filtering through a 0.22μm filter membrane.

[0031] Preparation:

[0032] 1) Take about 3ml of canine placenta, wash it with PBS containing 10% double antibody, use sterilized ophthalmic scissors to cut the placenta into 1mm size, enzymolyze it with type Ⅰ collagenase in a 37°C incubator for 40min, and sterilize Filter the single-cell filter membrane, centrifuge at 1200r / min for 5min to remove the supernatant, resuspend the cells with complete ...

Embodiment 3

[0036] An eye drop containing canine mesenchymal stem cell factor, which consists of 55% supernatant of canine amniotic fluid mesenchymal stem cell culture medium, 0.15% chloramphenicol, 0.65% sodium hyaluronate, polyethylene glycol 800 3%, 1,2-propanediol 5%, and the balance of normal saline.

[0037] As a further improvement of the above scheme, the supernatant of the canine mesenchymal stem cell culture medium is obtained by collecting the supernatant of canine mesenchymal stem cells through cell culture, centrifuging at 4000r / min for 20min, and filtering through a 0.22μm filter membrane.

[0038] Preparation:

[0039] 1) Take about 3ml of canine amniotic fluid, hydrolyze it with type I collagenase in a 37°C incubator for 40min, sieve through a sterilized single-cell filter membrane, centrifuge at 1200r / min for 5min to remove the supernatant, and resuspend the cells in complete medium. Add appropriate amount of complete medium to a petri dish and culture in a 5% carbon dio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an eye drop containing canine mesenchymal stem cell factor, which comprises 40-55% of canine mesenchymal stem cell culture supernatant, 0.15-0.28% of chloramphenicol, sodium hyaluronate 0.25-0.65%, surfactant 3-8%, co-surfactant 1.5-5% and the balance of normal saline. The canine mesenchymal stem cell culture supernatant of the present invention contains a variety of active cytokines, which has the effects of promoting corneal injury repair, accelerating the healing speed of scar tissue, effectively reducing the generation of eye secretions after injury, and the compound formula makes dripping In addition to the repairing function, the eye drops also have excellent antibacterial ability, which effectively reduces the risk of secondary infection, and solves the problem of tearing eyes, increased eye secretions, shame, and difficulty in repairing scar tissue caused by severe corneal damage in dogs. total problem. The invention also discloses a preparation method of the eye drop.

Description

technical field [0001] The invention relates to the field of veterinary medicine, in particular to an eye drop. Background technique [0002] Mesenchymal stem cells (Adipose mesenchymal stem cells, AD-MSC) are ubiquitous in fat, bone marrow, umbilical cord, placenta, amniotic fluid and other tissues or body fluids. They have a wide range of sources, are easy to separate, and have self-renewal, tissue damage repair, and immune regulation. It also has the ability to differentiate into epidermal cells. [0003] The secretion of mesenchymal stem cells cultured in vitro contains a large number of active ingredients, such as stem cell growth factor (SCF), fibroblast growth factor (FGF), epidermal growth factor (EGF), cell colony stimulating factor, collagen, etc. 80 A variety of active ingredients, which have a certain effect on promoting the recovery of corneal damage. [0004] Canine corneal injury accounts for at least half of the serious eye injuries in dogs. If the canine c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/28A61K9/08A61K47/10A61P27/02A61K31/165A61K31/728
CPCA61K47/10A61P27/02A61K9/0048A61K9/08A61K31/165A61K31/728A61K35/28A61K2300/00
Inventor 陈淑仪陈胜锋王丙云陈志胜林嘉颖姚家威
Owner 维赛(佛山)生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com