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A method for cultivating Zizhi strain and a Zizhi liquid fermentation method

A method for culturing and a technology for strains, which are applied in the field of cultivating Zizhi strains and can solve the problems of low yield of Zizhi polysaccharides and the like.

Active Publication Date: 2022-01-07
BEIJING BRILLIANCE BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the defect in the prior art of cultivating the mycelium of Zizhi mycelium to obtain the low yield of polysaccharides of Zizhi, the present invention provides a method for cultivating mycelium of Zizhi and a liquid fermentation method of Zizhi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Cultivation of Zizhi strains in inclined test tubes:

[0047] Preparation of maple water extraction solution: mix maple branches (dry weight) with deionized water at a ratio of 7:100, boil for 20 minutes, and filter through gauze to obtain maple water extraction solution.

[0048] PDA medium (g / L): Potato 250g, add 500ml deionized water and boil for 30min, filter with gauze to get the filtrate; add glucose 30g, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 0.8g, VB 1 0.01g, 15g of agar, add the maple water extract solution prepared above to make up to 1L.

[0049] Bacteria culture stage: Dig a piece (1*1cm) of bacteria lawn from the storage test tube of Zizhi bacteria (China Agricultural Microorganism Culture Collection Management Center, preservation number: ACCC50045) and inoculate it in solid PDA medium for activation and rejuvenation. After inoculating the strains, culture them in an incubator at 24° C. for 4 days to obtain an activated strain of Zizhi that grows vigorousl...

Embodiment 2

[0058] 1. Cultivation of Zizhi strains in inclined test tubes:

[0059] Preparation of maple water extraction solution: mix maple branches (dry weight) with deionized water at a ratio of 5:100, boil for 50 min, and filter through gauze to obtain maple water extraction solution.

[0060] PDA medium (g / L): Potato 150g, add 500ml deionized water and boil for 30min, filter with gauze to get the filtrate; add glucose 50g, KH 2 PO 4 5g, MgSO 4 ·7H 2 O 0.5g, VB 1 0.03g, 30g agar, add the maple water extract solution prepared above, and dilute to 1L.

[0061] Bacteria culture stage: Dig a piece (1*1cm) of bacteria lawn from the storage test tube of Zizhi bacteria (China Agricultural Microorganism Culture Collection Management Center, preservation number: ACCC50045) and inoculate it in solid PDA medium for activation and rejuvenation. After inoculating the strains, culture them in an incubator at 24° C. for 4 days to obtain an activated strain of Zizhi that grows vigorously (ful...

Embodiment 3

[0071] 1. Cultivation of Zizhi strains in inclined test tubes:

[0072] Preparation of maple water extraction solution: mix maple branches (dry weight) with deionized water at a ratio of 7:100, boil for 40 minutes, and filter through gauze to obtain maple water extraction solution.

[0073] PDA medium (g / L): Potato 300g, add 500ml deionized water and boil for 30min, filter with gauze to get the filtrate; add glucose 10g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, VB 1 0.01g, 5g of agar, add the maple water extract solution prepared above, and dilute to 1L.

[0074] Bacteria culture stage: Dig a piece (1*1cm) of bacteria lawn from the storage test tube of Zizhi bacteria (China Agricultural Microorganism Culture Collection Management Center, preservation number: ACCC50045) and inoculate it in solid PDA medium for activation and rejuvenation. After inoculating the strains, culture them in an incubator at 24° C. for 4 days to obtain an activated strain of Zizhi that grows vigorousl...

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Abstract

The invention provides a method for cultivating Zizhi strains, wherein, in the stage of test tube culture, the test tube culture medium includes: nitrogen source, carbon source, phosphate, sulfate, agar, and maple water extraction solution. The present invention also provides a method for cultivating Zizhi bacterial strains and a Zizhi liquid fermentation method, wherein the shake flask seed culture liquid and / or fermentation culture liquid include nitrogen source, carbon source, phosphate, sulfate, and maple water Extract the solution. The inventors of the present application unexpectedly found that for the Zizhi strains that propagate wildly on maple trees, if a certain concentration of maple water extract solution is added to the culture medium as a nutritional supplement during the artificial cultivation process, the growth and development of Zizhi can be greatly promoted. It promotes the secretion of the effective components of Zizhi. For example, with the same inoculation amount and the same capacity of the fermenter, the dry weight of mycelium obtained can be increased by at least 1.5 times; the polysaccharide of Zizhi obtained from the fermentation broth can be increased by at least 1.6 times.

Description

technical field [0001] The invention relates to a method for cultivating Zizhi bacteria strains and a Zizhi liquid fermentation method. Background technique [0002] Zizhi is a fine product of traditional Chinese ganoderma. The active ingredients of Zizhi mainly include Zizhi polysaccharides and triterpenoids (ganoderic acid). The medicinal value of Zizhi polysaccharide mainly includes anti-cancer, hypoglycemic, immune regulation, and promotion of protein and nucleic acid synthesis; the medicinal value of ganoderma acid mainly includes liver protection and detoxification, lowering cholesterol, inhibiting the release of histamine, and inhibiting vascular tension Peptide conversion, etc. [0003] Zizhi is difficult to domesticate and cultivate artificially, so Zizhi products are rarely seen in the market. Studies have found that the mycelium of Zizhi also contains a large amount of active ingredients of Zizhi, such as Zizhi polysaccharide. Therefore, people began to try to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/645
CPCC12N1/14C12P19/04
Inventor 张雷杨淑琴郭秀茹
Owner BEIJING BRILLIANCE BIOCHEM
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