The invention provides a method for cultivating Zizhi strains, wherein, in the stage of test tube culture, the test tube culture medium includes: nitrogen source, carbon source, phosphate, sulfate, agar, and maple water extraction solution. The present invention also provides a method for cultivating Zizhi bacterial strains and a Zizhi liquid fermentation method, wherein the shake flask seed culture liquid and / or fermentation culture liquid include nitrogen source, carbon source, phosphate, sulfate, and maple water Extract the solution. The inventors of the present application unexpectedly found that for the Zizhi strains that propagate wildly on maple trees, if a certain concentration of maple water extract solution is added to the culture medium as a nutritional supplement during the artificial cultivation process, the growth and development of Zizhi can be greatly promoted. It promotes the secretion of the effective components of Zizhi. For example, with the same inoculation amount and the same capacity of the fermenter, the dry weight of mycelium obtained can be increased by at least 1.5 times; the polysaccharide of Zizhi obtained from the fermentation broth can be increased by at least 1.6 times.