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A kind of multi-linked dry powder inactivated vaccine of clostridium sheepis-abamectin compound preparation

A technology of abamectin and compound preparations, which is applied in the direction of vaccines, veterinary vaccines, antibacterial drugs, etc., and can solve problems such as wasting material and financial resources, disturbing animals, and animal stress

Active Publication Date: 2020-10-30
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The prevention and treatment of Clostridium ammonium disease and gastrointestinal nematode disease are usually carried out separately. In actual work, it will not only disturb the animals and cause animal stress; it will also bring a lot of inconvenience and trouble to the staff, wasting material and financial resources

Method used

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  • A kind of multi-linked dry powder inactivated vaccine of clostridium sheepis-abamectin compound preparation
  • A kind of multi-linked dry powder inactivated vaccine of clostridium sheepis-abamectin compound preparation
  • A kind of multi-linked dry powder inactivated vaccine of clostridium sheepis-abamectin compound preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Accurately weigh 0.100g of avermectin powder into a sterilized ampoule, and add 0.75g of toluene and 0.25g of co-solvent DMF into it. After the drug is completely dissolved, add compound emulsifier Span-20 0.1g and Tween-20 0.3g. Mix well to form the oil phase.

[0048] 2. Take 8.0 g of 20% aluminum hydroxide physiological saline, add 0.05 g of thickener (sodium carboxymethyl cellulose) and 0.4 g of antifreeze agent (propylene glycol) into it, and mix well to form the water phase.

[0049] 3. Drop the oil phase into the water phase, and stir with a high-shear emulsifier at 12,000 rpm for 10 minutes in a sterile state to obtain a well-dispersed abamectin-in-water emulsion (EW);

[0050] 4. Take 1mL of abamectin aqueous emulsion and 1 dose of Clostridium hiatus dry powder vaccine, shake and mix well to obtain a compound preparation.

Embodiment 2

[0051] The development and quality analysis of embodiment 2 clostridioides disease multi-linked dry powder inactivated vaccine-abamectin compound preparation

[0052] 1 Materials and methods

[0053] 1.1 Materials

[0054] 1.1.1 Main equipment and instruments

[0055] Y10 high-shear emulsifier, Shanghai Yicheng Electromechanical Co., Ltd.; AY-120 analytical balance, Japan Shimadzu Corporation; Rise-2006 wet laser particle size analyzer, Jinan Runzhi Technology Co., Ltd.; LC-20AT high-efficiency Liquid chromatograph, Japan Shimadzu Corporation; KQ-700DV CNC ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd.; SHB-Ⅲ circulating water multi-purpose vacuum pump, Zhengzhou Century Shuangke Experimental Instrument Co., Ltd.; single-channel pipette , Thermo Fisher Scientific (China) Co., Ltd.; PHS-25C acidity meter, Shanghai Yulong Instrument Co., Ltd.; rotary viscometer, Shanghai Hongji Instrument Equipment Co., Ltd.; DHG-9240 blast drying oven, Shanghai No. 1 Heng Scienc...

Embodiment 3

[0285] The immunological research of embodiment 3 clostridium hirsutum multi-linked dry powder inactivated vaccine-abamectin compound preparation

[0286] 1 Immunological potency detection of compound preparation

[0287] 1.1 Rabbit serum antibody detection

[0288] Six big-eared rabbits were taken, numbered 1-6, and 5 mL of blood was collected from each animal before injection, the serum was separated, and stored at -20°C for future use. Rabbits 1 to 4 were the test group, and each rabbit was subcutaneously injected with 1 mL of the compound preparation. Rabbit No. 5 was injected with 1 mL of vaccine subcutaneously as a positive control, and rabbit No. 6 was injected with 1 mL of normal saline as a blank control. After 18 days, blood was collected, serum was separated, and stored at -20°C for antibody detection.

[0289] Mix equal amounts of No. 1 to No. 4 sera, and take 0.4 mL of the mixed serum and 0.8 mL of Clostridium putrefaciens toxin (containing 4 mouse MLDs) and Clo...

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Abstract

The invention relates to animal vaccines, and in particular discloses a sheep clostridial disease polyvalent dry powder inactivated vaccine-abamectin composite preparation which comprises 1% of abamectin, 5-10% of a composite solvent, 3-6% of an emulsifier, 0.2-2% of a thickening agent, 0-10% of an anti-freezing agent and the balance of 20% aluminum hydroxide normal saline, wherein the concentration of a sheep clostridial disease dry powder vaccine in the composite preparation is 1 sheep / mL. An abamectin emulsion in water is prepared by using a phase transfer method. Screening results of a single solvent, a composite solvent, a surfactant, an anti-freezing agent and a thickening agent of the emulsion in water are comprehensively analyzed, and an abamectin emulsion in water with good dispersibility is provided. On the basis, the sheep clostridial disease polyvalent dry powder inactivated vaccine-abamectin composite preparation is provided, and simultaneous prevention and treatment on sheep clostridial diseases gastrointestinal nematodiasis are achieved. In actual application, animal stress caused by animal disturbance is reduced, operation inconvenience of operators is reduced, and the application cost is lowered.

Description

technical field [0001] The invention relates to an animal vaccine, in particular to a multi-combined dry powder inactivated vaccine-abamectin compound preparation of clostridioides. Background technique [0002] Clostridia is a group of diseases caused by microorganisms of the genus Clostridium. It mainly includes sheep rapid epidemic, sheep enterotoxemia, sheep sudden attack, and lamb dysentery. Sheep plague is an acute infectious disease that mainly occurs in sheep. The onset is sudden, the course of the disease is very short, and the abomasum is hemorrhagic and inflammatory. Sheep enterotoxemia has acute onset and rapid death, and softening of the kidneys is common after death. The sudden onset of sheep is rapid, with diarrhea, muscle cramps, falling to the ground, spasms of limbs, and often sudden death. Lamb dysentery is an acute toxemia of newborn lambs, characterized by severe diarrhea and ulceration of the small intestine, which can often cause mass death of lambs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/08A61K9/107A61K47/06A61K47/18A61K47/22A61K47/26A61K47/38A61K47/10A61P31/04A61P33/10A61K31/7048
CPCA61K9/107A61K31/7048A61K39/08A61K47/06A61K47/10A61K47/18A61K47/22A61K47/26A61K47/38A61K2039/521A61K2039/552A61K2300/00
Inventor 杨晓野李莹莹王瑞李斌陈林军
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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