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A largemouth bass rhabdovirus yolk antibody

A yolk antibody, largemouth bass technology, applied in the direction of antibodies, antiviral agents, antiviral immunoglobulins, etc., can solve the problems of imperfect development of the immune system of fry, and difficulty in achieving active immune prevention.

Active Publication Date: 2021-07-06
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the rhabdovirus mainly harms perch fry below 5cm, and the immune system of fry at this stage is not fully developed, active immune prevention is difficult to achieve

Method used

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  • A largemouth bass rhabdovirus yolk antibody
  • A largemouth bass rhabdovirus yolk antibody
  • A largemouth bass rhabdovirus yolk antibody

Examples

Experimental program
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Embodiment

[0029] A largemouth bass rhabdovirus yolk antibody prepared by the following method:

[0030] 1. Isolation and culture of rhabdovirus from largemouth bass

[0031] Get the largemouth bass fry (from the breeding factory in Huzhou City, Zhejiang Province, body length 2-5cm) infected with MSRV, freeze and thaw twice after homogenization, centrifuge 30min at 12000rpm, get the supernatant and filter through a 0.25 μm bacterial filter, The filtrate was the crude virus extract, which was aliquoted and stored at -80°C for future use.

[0032] Grass carp ovary cell line CO cells were used as sensitive cells for largemouth bass rhabdovirus. The culture medium for CO cells was M199 complete medium (10% fetal bovine serum, 100 μg / mL penicillin, and 100 μg / mL streptomycin were added to M199 basal medium). Cultivate in a cell culture flask of T25 specification, add 4-5 milliliters of M199 complete medium, when the CO cells are at a confluence of 60-80%, suck out the culture solution, add ...

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Abstract

The present invention discloses a largemouth bass rhabdovirus yolk antibody, comprising: (1) separating and culturing largemouth rhabdovirus from largemouth rhabdovirus infected with largemouth bass; (1) obtaining largemouth rhabdovirus; ( 2) inactivating largemouth bass rhabdovirus; (3) immunizing 180-190-day-old laying hens with the inactivated largemouth bass rhabdovirus as antigen, collecting the eggs of laying hens after immunization, Extraction and purification of yolk antibody from egg yolk. The invention effectively neutralizes the largemouth bass rhabdovirus, thereby terminating the virus infection, providing an effective passive immune preparation for subsequent use in the prevention and control of the virus, and for reducing the virus-carrying rate of the parent and improving the survival rate of the seedlings of great significance.

Description

technical field [0001] The invention relates to the technical field of production of fish biological products, in particular to a largemouth bass rhabdovirus yolk antibody. Background technique [0002] Largemouth bass Micropterus salmoides, commonly known as California perch, is native to the Mississippi River system in California, USA. It has delicious meat and is very popular in the market. In addition, it has the characteristics of rapid growth, easy to catch, wide temperature range, and strong low oxygen resistance. Very popular with farmers. my country's annual output can reach more than 300,000 tons, which is the country with the highest output in the world. In 2016, the breeding area of ​​largemouth bass in Zhejiang Province was nearly 50,000 mu. In recent years, California perch has become the main species of raceway recirculating aquaculture. However, whether it is traditional high-density breeding or track-style centralized captivity, there is a potential dange...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C07K16/02A61K39/42A61P31/14
CPCA61K2039/505A61P31/14C07K16/10C07K2317/11
Inventor 刘莉吕孙建袁雪梅施伟达杭小英吴颖蕾
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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