Immersion device for dynamic adaptation of a medium to a sample and method

一种介质、样品的技术，应用在显微镜领域，能够解决折射率与样品的折射率失调、显微成像限制等问题，达到减小像差的效果

Active Publication Date: 2019-04-05
CARL ZEISS MICROSCOPY GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The present invention should solve the problem of limitations of microscopic imaging using immersion objectives due to misalignment between the refractive index of the immersion medium and that of the sample, especially for live cell microscopy

Method used

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  • Immersion device for dynamic adaptation of a medium to a sample and method
  • Immersion device for dynamic adaptation of a medium to a sample and method
  • Immersion device for dynamic adaptation of a medium to a sample and method

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Embodiment Construction

[0060] already on Figure 1a to Figure 1c The described elements are also present in other exemplary embodiments. Here, the same reference numerals refer to the same technical elements.

[0061] As essential technical elements, the arrangement according to the invention comprises a medium supply unit 1, a mixing device 2, an image capture unit 16, an evaluation unit 17 and a control unit 18 ( figure 2 ).

[0062] The medium supply unit 1 comprises a mixing device 2, from which a first component K of a medium 6 can be mixed by means of a pump 3 1 and the second component K 2 to the mixing plant 2. The medium supply unit 1 is connected via a medium line 4 to a first storage container 5.1 and a second storage container 5.2, wherein the first component K 1 Stored in the first storage container 5.1, and the second component K 2 Stored in the second storage container 5.2. The first component K 1 has a refractive index n 1 , while the second component K 2 has a refractive in...

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Abstract

An arrangement for supplying immersion media. The arrangement includes a media supply unit for the controlled supply of a medium or of a mixture into a contact region between an optical lens and a specimen slide, on which a specimen may be arranged. An image capture unit is provided for capturing image data on the basis of detection radiation from the object space along a detection beam path extending through the contact region. An evaluation unit is provided to establish current image parameters on the basis of captured image data, to compare said current image parameters to intended image parameters and to establish a desired mixing ratio of at least two components of the medium depending on the comparison. A mixing apparatus for the controlled setting of the mixing ratio of the components of the medium to be introduced into the contact region and a control unit for actuating the mixing apparatus depending on the established desired mixing ratio are parts of the arrangement. In addition, the invention relates to a method for setting optical parameters of a medium.

Description

technical field [0001] The invention relates to an arrangement and a method for setting the optical properties of a medium, in particular an immersion medium, and to a microscope comprising such an arrangement. Background technique [0002] Especially in live cell microscopy, the precise refractive index of the sample is often not known. This does not (only) concern the embedding medium and / or the buffer employed, but more particularly the interior of the sample, which may be provided by eg cells, (tumor) spheroids, tissue sections or whole organisms. [0003] Because these samples are usually stored and examined in aqueous solutions (buffered solutions), water immersion objectives are often used - also for historical reasons. These are optimized for a refractive index of n = 1.33 (refractive index of water). However, the refractive index of living cell samples deviates from n = 1.33 (see for example, Liu, P.Y. et al. "Cell refractive index for cell biology and disease dia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/41G01N21/64G01N21/84G01N1/38G02B21/33G02B21/34G02B21/02
CPCG01N1/38G01N21/01G01N21/41G01N21/64G01N21/84G02B21/02G02B21/33G02B21/34G02B21/365H04N23/60G05D11/02G06T7/0014G06T2207/10056G06T2207/30024G06T2207/30168
Inventor T.卡尔克布伦纳S.博尔克J.西本摩根
Owner CARL ZEISS MICROSCOPY GMBH
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