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Optical measuring system and method for cell perforation

An optical measurement system and cell technology, applied in the field of optical measurement system for cell perforation, can solve the problem of high cost of femtosecond lasers, and achieve the effect of low cost, high spatial resolution and time resolution

Active Publication Date: 2019-04-12
XI AN JIAOTONG UNIV
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Problems solved by technology

The existing photoporation method mainly uses femtosecond laser to excite cells to produce perforation, but the cost of femtosecond laser is very expensive, which is the main disadvantage that limits the application of photoporation in the field of gene transfection

Method used

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  • Optical measuring system and method for cell perforation
  • Optical measuring system and method for cell perforation
  • Optical measuring system and method for cell perforation

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Embodiment Construction

[0036] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. Apparently, the described embodiments are some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0037] Such as figure 1 As shown, an optical measurement system for cell perforation includes a three-dimensional motion platform 4, a cell culture dish 3 is arranged on the three-dimensional motion platform 4, a circular hole with a diameter of 40 mm is arranged in the center of the three-dimensional motion platform 4, and a mirror 2 is installed on a vertical Directly above the circular hole of the three-dimensional motion platform 4, the angle between the mirror 2 and the vertical direction perpendicu...

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Abstract

The invention relates to optical measuring system and method for cell perforation. A cell culture dish and a reflecting mirror are arranged on a three-dimensional moving platform; a 642nm laser deviceis arranged on the horizontal right side of the reflecting mirror; an objective lens, an A dichroscope, a B dichroscope, a C dichroscope and a photoelectric detection device are sequentially arrangedright under the three-dimensional moving platform; two focusing lenses are arranged in the horizontal rightward direction of the A dichroscope; laser devices are arranged on the sides of the focusinglenses and the dichroscopes and are connected with a delay device; and a high-speed camera is arranged on the right side of one dichroscope and is connected with the photoelectric detection device are connected with a digital signal processor (DSP) processing system. The method comprises the steps of culturing a cell in the cell culture dish, and adding nanometer gold and a voltage-sensitive dye;using the laser devices for continuously irradiating the cell and exciting the voltage-sensitive dye; using the high-speed camera for acquiring an image; and calculating and drawing a variation trendof a cell span membrane potential through the DSP processing system. According to the optical measuring system and the method for cell perforation provided by the invention, the cell can be recoverably perforated at low cost.

Description

technical field [0001] The invention relates to the technical field of cell optical perforation and biological tissue potential measurement, in particular to an optical measurement system and method for cell perforation. Background technique [0002] Gene therapy is a promising and effective method in the treatment of diseases that are difficult to cure by drugs (such as cancer, AIDS, genetic diseases, etc.). Gene therapy is to load exogenous drugs or genes into cells, and fundamentally realize the treatment of diseases. In this process, reversible perforation of cells is a key step in the realization of gene therapy. [0003] Traditional cell perforation methods include biological methods, chemical methods, mechanical methods and physical methods. However, biological methods are potentially toxic and can easily infect healthy cells around the target cells; chemical methods have relatively low transfection efficiency; mechanical methods require particularly high operationa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01B11/00G01N27/00
CPCG01B11/00G01N27/00
Inventor 姚翠萍杜晓凡王晶张镇西王斯佳辛静
Owner XI AN JIAOTONG UNIV
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